MoMCP1, a Cytochrome P450 Gene, Is Required for Alleviating Manganese Toxin Revealed by Transcriptomics Analysis in Magnaporthe oryzae

Int J Mol Sci. 2019 Mar 29;20(7):1590. doi: 10.3390/ijms20071590.


Manganese, as an essential trace element, participates in many physiological reactions by regulating Mn associated enzymes. Magnaporthe oryzae is a serious pathogen and causes destructive losses for rice production. We identified a cytochrome P450 gene, MoMCP1, involving the alleviation of manganese toxin and pathogenicity. To identify the underlying mechanisms, transcriptomics were performed. The results indicated that many pathogenicity related genes were regulated, especially hydrophobin related genes in ∆Momcp1. Furthermore, the Mn2+ toxicity decreased the expressions of genes involved in the oxidative phosphorylation and energy production, and increased the reactive oxygen species (ROS) levels, which might impair the functions of mitochondrion and vacuole, compromising the pathogenicity and development in ∆Momcp1. Additionally, our results provided further information about Mn associated the gene network for Mn metabolism in cells.

Keywords: Magnaporthe oryzae; manganese toxin; transcriptomics.

MeSH terms

  • Cytochrome P-450 Enzyme System / genetics*
  • Fungal Proteins / genetics*
  • Fungal Proteins / metabolism
  • Gene Expression Profiling*
  • Gene Expression Regulation, Fungal
  • Genes, Fungal*
  • Green Fluorescent Proteins / metabolism
  • Hydrogen Peroxide / metabolism
  • Hyphae / metabolism
  • Intracellular Space / metabolism
  • Magnaporthe / genetics*
  • Magnaporthe / pathogenicity
  • Manganese / toxicity*
  • Mutation / genetics
  • Oryza / microbiology
  • Superoxide Dismutase / metabolism
  • Transcriptome / genetics


  • Fungal Proteins
  • Green Fluorescent Proteins
  • Manganese
  • Cytochrome P-450 Enzyme System
  • Hydrogen Peroxide
  • Superoxide Dismutase