Evaluation of Toll-like receptor expression profile in patients with psoriasis vulgaris

Gene. 2019 Jun 20:702:166-170. doi: 10.1016/j.gene.2019.03.058. Epub 2019 Mar 29.

Abstract

TLRs are thought to play a role in the pathophysiology of such dermatological diseases as leprosy, acne and psoriasis. The study included 20 patients with plaque psoriasis, as well as 20 healthy age- and gender-matched control subjects. Real-time polymerase chain reaction evaluation was made of the messenger RNA expression of TLRs 1-10 in lesional tissue and peripheral blood mononuclear cell samples in psoriasis patients. TLR 3, 5, 6, 7, 9 and 10 lesional tissue mRNA expressions were increased significantly when compared to the expression levels in the PBMCs of the same patients (p = 0.0082, p = 0.0176, p = 0.0239, p = 0.0261, p = 0.0223, p = 0.0206). A comparison of the TLR expression in the PBMCs of healthy subjects and the PBMCs of patients with psoriasis showed a significant increase in the TLR 1, 8 and 10 mRNA expressions in the patient group (p < 0.0001, p < 0.0001, p = 0.0035). The TLR 5 mRNA expression was significantly higher in the control group than in the patient group (p = 0.0037). To the best of our knowledge, this is the first study in literature to evaluate mRNA TLR expression levels in the lesional tissue and PBMCs of patients with psoriasis.

Keywords: PBMC; Psoriasis; TLRs, mRNA expression.

MeSH terms

  • Adult
  • Female
  • Gene Expression
  • Humans
  • Leukocytes, Mononuclear / metabolism
  • Male
  • Middle Aged
  • Psoriasis / blood
  • Psoriasis / genetics
  • Psoriasis / metabolism*
  • Toll-Like Receptor 1 / blood
  • Toll-Like Receptor 1 / genetics
  • Toll-Like Receptor 10 / blood
  • Toll-Like Receptor 10 / genetics
  • Toll-Like Receptor 8 / blood
  • Toll-Like Receptor 8 / genetics
  • Toll-Like Receptors / blood
  • Toll-Like Receptors / genetics
  • Toll-Like Receptors / metabolism*

Substances

  • TLR10 protein, human
  • TLR8 protein, human
  • Toll-Like Receptor 1
  • Toll-Like Receptor 10
  • Toll-Like Receptor 8
  • Toll-Like Receptors