An orange calcium-modulated bioluminescent indicator for non-invasive activity imaging

Nat Chem Biol. 2019 May;15(5):433-436. doi: 10.1038/s41589-019-0256-z. Epub 2019 Apr 1.


Fluorescent indicators are used widely to visualize calcium dynamics downstream of membrane depolarization or G-protein-coupled receptor activation, but are poorly suited for non-invasive imaging in mammals. Here, we report a bright calcium-modulated bioluminescent indicator named Orange CaMBI (Orange Calcium-modulated Bioluminescent Indicator). Orange CaMBI reports calcium dynamics in single cells and, in the context of a transgenic mouse, reveals calcium oscillations in whole organs in an entirely non-invasive manner.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calcium / chemistry*
  • Luminescent Measurements
  • Luminescent Proteins / chemistry*
  • Mice
  • Mice, Transgenic
  • Optical Imaging*
  • Organometallic Compounds / chemistry*


  • Luminescent Proteins
  • Organometallic Compounds
  • Calcium