Transcriptome-Wide Mapping 5-Methylcytosine by m 5 C RNA Immunoprecipitation Followed by Deep Sequencing in Plant

Methods Mol Biol. 2019;1933:389-394. doi: 10.1007/978-1-4939-9045-0_24.

Abstract

Transcriptome-wide mapping RNA modification is crucial to understand the distribution and function of RNA modifications. Here, we describe a protocol to transcriptome-wide mapping 5-methylcytosine (m5C) in plant, by a RNA immunoprecipitation followed by deep sequencing (m5C-RIP-seq) approach. The procedure includes RNA extraction, fragmentation, RNA immunoprecipitation, and library construction.

Keywords: 5-Methylcytosine; Plant; RNA immunoprecipitation; RNA methylation; m5C; m5C-RIP-seq.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 5-Methylcytosine / chemistry*
  • Arabidopsis / genetics*
  • Computational Biology
  • Genome, Plant*
  • High-Throughput Nucleotide Sequencing / methods*
  • Immunoprecipitation / methods*
  • RNA, Plant / chemistry
  • RNA, Plant / genetics*
  • Sequence Analysis, RNA / methods
  • Transcriptome*

Substances

  • RNA, Plant
  • 5-Methylcytosine