Determination of kansuiphorin C and kansuinin A in rat feces using UFLC-MS/MS and its application in the comparative excretion study on normal and malignant ascites rats
- PMID: 30947126
- DOI: 10.1016/j.jpba.2019.03.054
Determination of kansuiphorin C and kansuinin A in rat feces using UFLC-MS/MS and its application in the comparative excretion study on normal and malignant ascites rats
Abstract
Malignant ascites (MA) is one of the severe complications of gastrointestinal tumors, affecting the patients' survival time and quality of life. Euphorbia kansui is a commonly used toxic Chinese herbal medicine for malignant ascites. Our previous study showed that the biological and toxicological effects of kansui were closely related to the gastrointestinal tract. The ingenane-type and jastrophane-type diterpenoids are both toxic and active components of kansui. The contents of kansuiphorin C (KPC) and kansuinin A (KA) take highest accounts in each type of diterpene. Hence, in this study, the efficacy and toxicity of KPC and KA on normal rats and MA rats were firstly evaluated by serum liver enzymes (ALT and AST), oxidative damage indicators (GSH, SOD, MDA and LDH), inflammatory indexes (TNF-α, IFN-γ and IL-2) and the volume of ascites. Changes in the levels of these indices showed that although the toxicity of KPC on normal rats was stronger than KA, KPC exhibited better efficacy to the malignant ascites with no obvious side effects at the dose of 10 mg·kg-1. Then, accurate and reliable methods for the determination of KPC and KA in the rat feces by ultra-fast liquid chromatography coupled with MS/MS detector (UFLC-MS/MS) were established, detected by the multiple reaction monitoring mode. The chromatographic separation was conducted on an XBbridge C18 column (50 mm × 2.1 mm, 2.5 μm) using gradient elution composed of 0.1% formic acid in water and acetonitrile. The flow rate was 0.5 mL·min-1 and column temperature was 30 °C. The method was finally applied to the comparative study on normal and malignant ascites rats given KPC and KA, respectively. Interestingly, the results showed that KPC's accumulative fecal excretion rate (normal, 19.22%±5.36%; model, 15.96%±3.47%) were much higher than that of KA (normal, 2.928%±0.741%; model, 2.835%±0.873%) at the same dose within 48 h. This suggested KPC had higher in-vivo transformations in comparison with KA, providing guidance for the further preclinical research of KPC and KA as promising compounds treating MA.
Keywords: Excretion; Kansuinin A; Kansuiphorin C; Malignant ascites; Toxicity; UFLC-MS/MS.
Copyright © 2019 Elsevier B.V. All rights reserved.
Similar articles
-
Kansuiphorin C and Kansuinin A ameliorate malignant ascites by modulating gut microbiota and related metabolic functions.J Ethnopharmacol. 2020 Mar 1;249:112423. doi: 10.1016/j.jep.2019.112423. Epub 2019 Nov 22. J Ethnopharmacol. 2020. PMID: 31765764
-
Comparison of content-toxicity-activity of six ingenane-type diterpenoids between Euphorbia kansui before and after stir-fried with vinegar by using UFLC-MS/MS, zebrafish embryos and HT-29 cells.J Pharm Biomed Anal. 2021 Feb 20;195:113828. doi: 10.1016/j.jpba.2020.113828. Epub 2020 Dec 7. J Pharm Biomed Anal. 2021. PMID: 33349474
-
The toxicity and efficacy evaluation of different fractions of Kansui fry-baked with vinegar on Walker-256 tumor-bearing malignant ascites effusion rats and normal rats.J Ethnopharmacol. 2018 Jun 12;219:257-268. doi: 10.1016/j.jep.2018.03.010. Epub 2018 Mar 17. J Ethnopharmacol. 2018. PMID: 29559373
-
The Chemical and Biological Properties of Euphorbia kansui.Am J Chin Med. 2016;44(2):253-73. doi: 10.1142/S0192415X16500154. Am J Chin Med. 2016. PMID: 27080940 Review.
-
Effect of the vinegar-process on chemical compositions and biological activities of Euphorbia kansui: A review.J Ethnopharmacol. 2020 Apr 24;252:112557. doi: 10.1016/j.jep.2020.112557. Epub 2020 Jan 10. J Ethnopharmacol. 2020. PMID: 31931159 Review.
Cited by
-
Interferon-γ secreted by recruited Th1 cells in peritoneal cavity inhibits the formation of malignant ascites.Cell Death Discov. 2023 Jan 23;9(1):25. doi: 10.1038/s41420-023-01312-5. Cell Death Discov. 2023. PMID: 36690649 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical
Miscellaneous
