Immobilization of Aspergillus quadrilineatus RSNK-1 multi-enzymatic system for fruit juice treatment and mannooligosaccharide generation

Food Chem. 2019 Aug 15;289:95-102. doi: 10.1016/j.foodchem.2019.03.035. Epub 2019 Mar 12.

Abstract

Aspergillus quadrilineatus RSNK-1 produced a multi-enzymatic system containing (U/gds) β-mannanase (1021), endo-xylanase (1 9 1), α-galactosidase (3.42), β-xylosidase (0.07) and β-glucosidase (0.28) on low-cost copra meal (CM) in SSF. The enzyme preparation was covalently immobilized on aluminum oxide pellets (3 mm) under statistically optimized conditions leading to 73.17% immobilization yield. The immobilized enzyme (Man-AOP) displayed enhanced thermal and pH stability. Man-AOP was characterized by FTIR, SEM and PXRD revealing a covalent interaction. The bio-conjugate was successfully recycled for mannooligosaccharide (MOS) generation from locust bean gum (LBG) up to 10 cycles, yielding an average of 0.95 mg MOS/cycle. Man-AOP was also effective in clarification of apple, kiwi, orange and peach juices and enhanced their reducing sugar content. The bio-conjugate was useful in generation of MOS from mannan and enrichment of fruit juices.

Keywords: Aspergillus quadrilineatus; Fruit juice clarification; Immobilization; Locust bean gum; Mannooligosaccharide; β-Mannanase.

MeSH terms

  • Aluminum Oxide / chemistry
  • Aspergillus / enzymology*
  • Enzyme Stability
  • Enzymes, Immobilized / chemistry
  • Enzymes, Immobilized / metabolism
  • Fruit and Vegetable Juices / analysis*
  • Galactans / metabolism
  • Hydrogen-Ion Concentration
  • Mannans / metabolism
  • Oligosaccharides / metabolism*
  • Plant Gums / metabolism
  • Temperature
  • Xylosidases / chemistry
  • Xylosidases / metabolism
  • alpha-Galactosidase / chemistry
  • alpha-Galactosidase / metabolism
  • beta-Glucosidase / chemistry
  • beta-Glucosidase / metabolism
  • beta-Mannosidase / chemistry
  • beta-Mannosidase / metabolism

Substances

  • Enzymes, Immobilized
  • Galactans
  • Mannans
  • Oligosaccharides
  • Plant Gums
  • Xylosidases
  • beta-Glucosidase
  • alpha-Galactosidase
  • beta-Mannosidase
  • exo-1,4-beta-D-xylosidase
  • Aluminum Oxide
  • locust bean gum