Expression of CD24 in plasma, exosome and ovarian tissue samples of serous ovarian cancer patients

J Biotechnol. 2019 Jun 10:298:16-20. doi: 10.1016/j.jbiotec.2019.03.018. Epub 2019 Apr 5.


CD24 is a small molecular weight cell-surface protein and an independent marker for poor prognosis in the different type of cancers. We aimed to determine the expression of CD24 in plasma, exosomes and ovarian tissue samples of serous ovarian cancer patients. We collected tissue and blood samples from 21 cases of serous ovarian cancer and eight healthy controls. We used silica adsorption method for isolation of RNA. The cDNA was synthesized using quantitative real-time PCR. We used beta-globin as a housekeeping gene for the normalization of the data. Protein-protein and miRNA networking were analyzed. There was a significant difference in the expression of CD24 in ovarian tissue between controls and patients (0.16 ± 0.32 vs. 44.97 ± 68.06; p < 0.01), while CD24 did not show expression in each plasma and exosome samples. There was a correlation in the expression of CD24 and FIGO grading between controls and patients. CD24 expression was detected in exosomes in 38.1% of patients, mainly with FIGO III, and in their plasma in 9.5% of cases. Our network analysis shows LYN, SELP, FGR, and NPM1 proteins are interacting with CD24. Our study demonstrates higher expression of CD24 in ovarian cancer patients' tissue samples, and there is an association with FIGO classification. However, CD24 showed expression only in some cell-free plasma and exosome samples.

Keywords: CD24; Cell-free nucleic acids; Exosomes; Ovarian cancer; Plasma.

MeSH terms

  • Biomarkers, Tumor / genetics*
  • CD24 Antigen / genetics*
  • Cell Line, Tumor
  • Exosomes / genetics*
  • Exosomes / pathology
  • Female
  • Gene Expression Regulation, Neoplastic / genetics
  • Gene Regulatory Networks / genetics
  • Humans
  • MicroRNAs
  • Middle Aged
  • Neoplasm Proteins / genetics
  • Neoplasm Staging
  • Nucleophosmin
  • Ovarian Neoplasms / blood
  • Ovarian Neoplasms / genetics*
  • Ovarian Neoplasms / pathology
  • Ovary / metabolism
  • Ovary / pathology


  • Biomarkers, Tumor
  • CD24 Antigen
  • CD24 protein, human
  • MicroRNAs
  • NPM1 protein, human
  • Neoplasm Proteins
  • Nucleophosmin