Abstract
The aim of this study was to observe the effects of HIF-1α activation on myocardial I/R in diabetes. Diabetes was induced in an experimental rat model, and regulators of HIF-1α including KC7F2, deferoxamine and ginsenoside Rg1 were administered to observe the changes on diabetic rats. The results demonstrated that HIF-1α activation could effectively reduce myocardial injury following I/R in diabetic hearts via ERK but not MMP-2 signalling pathways. This activation promoted myocardial apoptosis, which was accompanied by modulation of Bax/Bcl-2, caspase-3 and caspase-9 expression following deferoxamine administration. Ginsenoside Rg1 application but not Re can activate HIF-1α, resulting in a similar protectively effect on these pathology processes. Our data demonstrated that ginsenoside Rg1 has a potential therapeutic effect by protecting diabetic hearts after myocardial injury following I/R via HIF-1α activation.
MeSH terms
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Animals
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Apoptosis / drug effects
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Caspase 3 / metabolism
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Caspase 9 / metabolism
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Deferoxamine / pharmacology
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Diabetes Mellitus, Experimental / chemically induced
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Diabetes Mellitus, Experimental / enzymology
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Diabetes Mellitus, Experimental / metabolism
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Extracellular Signal-Regulated MAP Kinases / metabolism
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Ginsenosides / chemistry
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Ginsenosides / pharmacology*
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Hypoxia-Inducible Factor 1, alpha Subunit / metabolism*
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MAP Kinase Signaling System / drug effects*
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Matrix Metalloproteinase 9 / metabolism
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Myocardial Infarction / drug therapy
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Myocardial Ischemia / drug therapy*
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Myocardial Ischemia / enzymology
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Myocardial Ischemia / metabolism
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Myocardial Ischemia / pathology
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Myocardial Reperfusion Injury / drug therapy*
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Myocardial Reperfusion Injury / enzymology
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Myocardial Reperfusion Injury / metabolism
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Myocardial Reperfusion Injury / pathology
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Proto-Oncogene Proteins c-bcl-2 / metabolism
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Rats
Substances
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Ginsenosides
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Hif1a protein, rat
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Hypoxia-Inducible Factor 1, alpha Subunit
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Proto-Oncogene Proteins c-bcl-2
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Extracellular Signal-Regulated MAP Kinases
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Caspase 3
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Caspase 9
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Matrix Metalloproteinase 9
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Mmp9 protein, rat
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Deferoxamine
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ginsenoside Rg1