Exploring sample cross-contamination in fish epidermal mucus

J Fish Biol. 2019 Aug;95(2):647-650. doi: 10.1111/jfb.13979. Epub 2019 Apr 30.

Abstract

Cross-contamination of epidermal mucus was assessed at three sampling locations on the bodies of Pacific halibut Hippoglossus stenolepis by inducing contact between fish coated with labelled synthetic mucus and non-treated fish. Results indicate a positive relationship between sampling site exposure and sample contamination and that mucous sample cross-contamination can be mitigated by sampling in a location protected from external contact.

Keywords: Hippoglossus stenolepis; Pacific halibut; cross-contamination; epidermal mucus.

MeSH terms

  • Alaska
  • Animal Fins / chemistry
  • Animals
  • Epidermis / chemistry*
  • Flounder / physiology*
  • Freezing
  • Lateral Line System / chemistry
  • Mucus / chemistry*
  • Specimen Handling / methods
  • Specimen Handling / standards
  • Specimen Handling / veterinary*