Effects of Photobiomodulation on Root Resorption Induced by Orthodontic Tooth Movement and RANKL/OPG Expression in Rats

Photochem Photobiol. 2019 Sep;95(5):1249-1257. doi: 10.1111/php.13107. Epub 2019 May 11.

Abstract

The aim of this study was to evaluate the effects of photobiomodulation on the repair of induced root resorption (RR) after orthodontic tooth movement. Twenty male rats were used in this study. Forty right and left upper first molars were evaluated and divided into four groups (n = 10): negative control group (NC), no tooth movement or irradiation; positive control group (PC), induced tooth movement and root resorption; conventional treatment group (CT), force was removed after 7 days; and photobiomodulation group (PBM) after force removal molars were irradiated every 48 h for 7 days using GaAlAs diode laser (810 nm). Energy per point was 1.5 J (100 mW, 15 s, 75 J cm-2 ). NC and PC were euthanized on day 7; CT and PBM on day 14. Histomorphometric and immunohistochemsitry analyses showed increase in area of root resorption in all groups (P < 0.05) compared to NC. RR lacunae were larger in CT compared to PC and PBM at the compression side of the distal root. OPG was higher in PBM group (P < 0.05). PBM group showed low expression of RANKL compared to PC and CT on the tension side. PBM can potentially affect RR progression by increasing OPG expression in the compression area and decreasing number of clastic cells in the root surface.

MeSH terms

  • Animals
  • Low-Level Light Therapy*
  • Male
  • Osteoprotegerin / metabolism*
  • RANK Ligand / metabolism*
  • Rats
  • Rats, Wistar
  • Root Resorption / prevention & control*
  • Tooth Movement Techniques*

Substances

  • Osteoprotegerin
  • RANK Ligand
  • Tnfrsf11b protein, rat