Role of Per3, a circadian clock gene, in embryonic development of mouse cerebral cortex

Sci Rep. 2019 Apr 10;9(1):5874. doi: 10.1038/s41598-019-42390-9.


Per3 is one of the primary components of circadian clock system. While circadian dysregulation is known to be involved in the pathogenesis of several neuropsychiatric diseases. It remains largely unknown whether they participate in embryonic brain development. Here, we examined the role of clock gene Per3 in the development of mouse cerebral cortex. In situ hybridization analysis revealed that Per3 is expressed in the developing mouse cortex. Acute knockdown of Per3 with in utero electroporation caused abnormal positioning of cortical neurons, which was rescued by RNAi-resistant Per3. Per3-deficient cells showed abnormal migration phenotypes, impaired axon extension and dendritic arbor formation. Taken together, Per3 was found to play a pivotal role in corticogenesis via regulation of excitatory neuron migration and synaptic network formation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Axons / physiology
  • Cell Movement
  • Cerebral Cortex / growth & development
  • Cerebral Cortex / metabolism*
  • Cerebral Cortex / pathology
  • Embryonic Development / genetics*
  • In Situ Hybridization, Fluorescence
  • Mice
  • Neurons / cytology
  • Neurons / metabolism
  • Period Circadian Proteins / antagonists & inhibitors
  • Period Circadian Proteins / genetics*
  • Period Circadian Proteins / metabolism
  • RNA Interference
  • RNA, Small Interfering / metabolism
  • Stem Cells / cytology
  • Stem Cells / metabolism
  • Time-Lapse Imaging


  • Per3 protein, mouse
  • Period Circadian Proteins
  • RNA, Small Interfering