In Vitro Antibiotic Synergism Against Ocular Fungal Isolates

Am J Ophthalmol. 1978 Sep;86(3):359-67. doi: 10.1016/0002-9394(78)90240-4.

Abstract

A microtiter method for the determination of fungal sensitivities was used to determine the minimal inhibitory and fungicidal concentrations of two antifungal agents, amphotericin B and natamycin, both alone and in combination with four different antibiotics: rifampin, gentamicin, clindamycin, and tetracycline. Synergism was defined as a fourfold or greater reduction in the minimal inhibitory concentration, minimal fungicidal concentration, or both, of the antifungal agent in the presence of antibiotic; antagonism was defined as fourfold or greater increase in the minimal inhibitory concentration, minimal fungicidal concentration, or both. Amphotericin B and rifampin were synergistic against the majority of organisms, but synergism between amphotericin B and other antibiotics was infrequent. Combinations of natamycin and rifampin or of natamycin and gentamicin were synergistic against the majority of Fusarium solani tested. Combinations of amphotericin B and tetracycline were antagonistic against 14% of the organisms.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amphotericin B / pharmacology*
  • Anti-Bacterial Agents / pharmacology*
  • Candidiasis / microbiology
  • Clindamycin / pharmacology
  • Drug Synergism
  • Eye / microbiology*
  • Fungi / drug effects*
  • Fusarium
  • Gentamicins / pharmacology
  • Humans
  • In Vitro Techniques
  • Microbial Sensitivity Tests
  • Natamycin / pharmacology*
  • Rifampin / pharmacology
  • Tetracyclines / pharmacology

Substances

  • Anti-Bacterial Agents
  • Gentamicins
  • Tetracyclines
  • Clindamycin
  • Amphotericin B
  • Natamycin
  • Rifampin