Mouse Embryo Culture for the Study of Neural Crest Cells

Methods Mol Biol. 2019;1976:107-119. doi: 10.1007/978-1-4939-9412-0_9.

Abstract

Mammalian development occurs in utero, which makes it difficult to study the diverse morphogenetic events of neural crest cell development in vivo. Analyses of fixed samples in conjunction with histological methods to evaluate the spatiotemporal roles of specific genes of interest only provide snapshots of mammalian neural crest cell development. This chapter describes methods for isolating and culturing mouse embryos and their organs in vitro, outside the mother, to facilitate real-time imaging and functional analyses of the dynamics of neural crest cell development.

Keywords: Development; Electroporation; Gene transfer; Lipofection; Live imaging; Mouse embryo; Neural crest cell; Roller culture; Whole embryo culture.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Differentiation / physiology
  • Cell Movement / physiology
  • Electroporation
  • Embryo Culture Techniques
  • Embryo, Mammalian / cytology
  • Mice
  • Neural Crest / cytology*