Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 96 (1), 129-138

Comprehensive Three-Dimensional Analysis (CUBIC-kidney) Visualizes Abnormal Renal Sympathetic Nerves After Ischemia/Reperfusion Injury

Affiliations

Comprehensive Three-Dimensional Analysis (CUBIC-kidney) Visualizes Abnormal Renal Sympathetic Nerves After Ischemia/Reperfusion Injury

Sho Hasegawa et al. Kidney Int.

Abstract

The sympathetic nervous system is critical in maintaining the homeostasis of renal functions. However, its three-dimensional (3D) structures in the kidney have not been elucidated due to limitation of conventional imaging methods. CUBIC (Clear, Unobstructed Brain/Body Imaging Cocktails and Computational analysis) is a newly developed tissue-clearing technique, which enables whole-organ 3D imaging without thin-sectioning. Comprehensive 3D imaging by CUBIC found that sympathetic nerves are primarily distributed around arteries in the mouse kidney. Notably, the sympathetic innervation density was significantly decreased 10 days after ischemia-reperfusion injury (voluminal ratio of innervation area to kidney) by about 70%. Moreover, norepinephrine levels in kidney tissue (output of sympathetic nerves) were significantly reduced in injured kidneys by 77%, confirming sympathetic denervation after ischemia-reperfusion injury. Time-course imaging indicated that innervation partially recovered although overall denervation persisted 28 days after injury, indicating a continuous sympathetic nervous abnormality during the progression of chronic kidney disease. Thus, CUBIC-kidney, the 3D imaging analysis, can be a strong imaging tool, providing comprehensive, macroscopic perspectives for kidney research.

Keywords: chronic kidney disease; ischemia-reperfusion injury; light-sheet fluorescent microscopy; sympathetic nerve; tissue clearing.

Similar articles

See all similar articles

Cited by 2 PubMed Central articles

LinkOut - more resources

Feedback