Quantitative RNA Analysis Using RNA-Seq

Methods Mol Biol. 2019:1971:95-108. doi: 10.1007/978-1-4939-9210-2_4.

Abstract

High-throughput sequencing of cDNA copies of mRNA (RNA-seq) provides a digital readout of mRNA levels over several orders of magnitude, as well as mapping the transcripts to the nucleotide level. Here we describe two different RNA-seq approaches, including one that exploits the 39-nucleotide mini-exon or spliced leader (SL) sequence found at the 5' end of all Leishmania (and other trypanosomatid) mRNAs.

Keywords: Differential gene expression; RNA-seq; Transcriptome; mRNA.

MeSH terms

  • Exons*
  • High-Throughput Nucleotide Sequencing / methods*
  • Leishmania / genetics*
  • RNA, Messenger / genetics*
  • RNA, Protozoan / genetics*
  • Sequence Analysis, RNA / methods*

Substances

  • RNA, Messenger
  • RNA, Protozoan