A chromosome-scale genome assembly and dense genetic map for Xenopus tropicalis

Dev Biol. 2019 Aug 1;452(1):8-20. doi: 10.1016/j.ydbio.2019.03.015. Epub 2019 Apr 10.


The Western clawed frog Xenopus tropicalis is a diploid model system for both frog genetics and developmental biology, complementary to the paleotetraploid X. laevis. Here we report a chromosome-scale assembly of the X. tropicalis genome, improving the previously published draft genome assembly through the use of new assembly algorithms, additional sequence data, and the addition of a dense genetic map. The improved genome enables the mapping of specific traits (e.g., the sex locus or Mendelian mutants) and the characterization of chromosome-scale synteny with other tetrapods. We also report an improved annotation of the genome that integrates deep transcriptome sequence from diverse tissues and stages. The exon-intron structures of these genes are highly conserved relative to both X. laevis and human, as are chromosomal linkages ("synteny") and local gene order. A network analysis of developmental gene expression will aid future studies.

Keywords: Comparative genomics; Gene expression analysis; Genetic mapping; Genome assembly; Pigmentation; Sex determination.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Chromosome Mapping*
  • Chromosomes / genetics*
  • Gene Expression Profiling*
  • Genome*
  • Humans
  • Molecular Sequence Annotation*
  • Xenopus