Expression of a Family of psbA Genes Encoding a Photosystem II Polypeptide in the Cyanobacterium Anacystis Nidulans R2

EMBO J. 1986 Nov;5(11):2789-98.

Abstract

The genome of the cyanobacterium Anacystis nidulans R2 contains three genes (psbA) for the QB protein of photosystem II. This protein is essential for oxygenic photosynthetic electron tansport, and is the target for several herbicides which act by binding directly to the photosynthetic apparatus. Transcripts from the three Anacystis psbA genes are present in wild-type cells at different steady-state levels. The nucleotide sequences of two of the genes, psbAII and psbAIII, predict a protein having the same amino acid sequence which differs from that of the psbAI gene by 25 (out of 360) residues. Inactivation of each of the psbA genes in the Anacystis chromosome, singly or in pairs, shows that each of the genes is capable of producing sufficient functional QB protein to support normal photoautotrophic growth.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Chlorophyll / genetics*
  • Cyanobacteria / genetics*
  • Cyanobacteria / metabolism
  • Genes*
  • Light-Harvesting Protein Complexes
  • Nucleic Acid Conformation
  • Photosynthesis
  • Photosynthetic Reaction Center Complex Proteins
  • Photosystem II Protein Complex
  • Plant Proteins / genetics*
  • Transcription, Genetic

Substances

  • Light-Harvesting Protein Complexes
  • Photosynthetic Reaction Center Complex Proteins
  • Photosystem II Protein Complex
  • Plant Proteins
  • Chlorophyll

Associated data

  • GENBANK/X04616
  • GENBANK/X04617
  • GENBANK/X04618