Biotin transport through the blood-brain barrier

J Neurochem. 1987 Feb;48(2):400-4. doi: 10.1111/j.1471-4159.1987.tb04107.x.


The unidirectional influx of biotin across cerebral capillaries, the anatomical locus of the blood-brain barrier, was measured with an in situ rat brain perfusion technique employing [3H]biotin. Biotin was transported across the blood-brain barrier by a saturable system with a one-half saturation concentration of approximately 100 microM. The permeability-surface area products were 10(-4) s-1 with a biotin concentration of 0.02 microM in the perfusate. Probenecid, pantothenic acid, and nonanoic acid but not biocytin or biotin methylester (all 250 microM) inhibited biotin transfer through the blood-brain barrier. The isolated rabbit choroid plexus was unable to concentrate [3H]biotin from medium containing 1 nM [3H]biotin. These observations provide evidence that: biotin is transported through the blood-brain barrier by a saturable transport system that depends on a free carboxylic acid group, and the choroid plexus is probably not involved in the transfer of biotin between blood and cerebrospinal fluid.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Biotin / metabolism*
  • Blood-Brain Barrier*
  • Capillary Permeability
  • Cerebrovascular Circulation
  • Lysine / analogs & derivatives
  • Lysine / pharmacology
  • Mathematics
  • Pantothenic Acid / pharmacology
  • Perfusion
  • Probenecid / pharmacology
  • Rats
  • Rats, Inbred Strains


  • Pantothenic Acid
  • Biotin
  • biocytin
  • Lysine
  • Probenecid