Modulation of immune response by bacterial lipopolysaccharide (LPS): cellular basis of stimulatory and inhibitory effects of LPS on the in vitro IgM antibody response to a T-dependent antigen

J Immunol. 1978 Dec;121(6):2347-51.

Abstract

The role of thymus-derived lymphocytes (T cells) in LPS modulation of T cell-dependent antibody responses has been investigated. We have assessed the effect of LPS on the primary anti-TNP response to TNP-SRBC of cultures of whole spleen cells or T cell-depleted spleen cells that were supplemented with various subpopulations of carrier-primed (SRBC) spleen cells. The TNP-PFC response was enhanced in the presence of irradiated SRBC-primed spleen cells by addition of 0.16 to 20 microgram/ml LPS, but inhibition was observed when irradiation of primed cells was omitted. Enhancement but no inhibition occurred when added primed cells were first passed through a nylon wool column. LPS-mediated enhancement was dependent on a T cell in the primed population. These results suggest that LPS modulation of antibody synthesis is dependent on two populations of antigen-specific cells that have opposing effects on B cell responses to a T-dependent antigen: a helper cell that is irradiation resistant, nonadherent to nylon wool, and sensitive to anti-T cell serum, and a suppressor cell that is irradiation sensitive and adherent to nylon wool.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Carrier Proteins / immunology
  • Dose-Response Relationship, Immunologic
  • Dose-Response Relationship, Radiation
  • Immunity, Cellular*
  • Immunoglobulin M / biosynthesis*
  • Lipopolysaccharides / pharmacology*
  • Mice
  • T-Lymphocytes / immunology*
  • T-Lymphocytes / radiation effects

Substances

  • Carrier Proteins
  • Immunoglobulin M
  • Lipopolysaccharides