Hd3a and OsFD1 negatively regulate rice resistance to Xanthomonas oryzae pv. oryzae and Xanthomonas oryzae pv. oryzicola

Biochem Biophys Res Commun. 2019 Jun 11;513(4):775-780. doi: 10.1016/j.bbrc.2019.03.169. Epub 2019 Apr 14.

Abstract

In rice, Hd3a, GF14 and OsFD1 proteins, forming florigen activation complex, are key components in flowering time regulation. GF14 genes in rice response to biotic and abiotic stress has also been well addressed. The role of GF14e in rice defense has been well studied. However, whether Hd3a and OsFD1 play roles in defense is unclear. In present study, we identified that Hd3a and OsFD1 expression is repressed by Xoo and JA, and validated that Hd3a and OsFD1 negatively regulate resistance to Xanthomonas oryzae pv. oryzae (Xoo) and Xanthomonas oryzae pv. oryzicola (Xoc). hd3a and osfd1 mutants increase resistance to Xoo and Xoc, and activate JA responsive genes expression. Our data also demonstrate that OsFD1 binds to the promoters of and activates the expression of JAZ genes; Hd3a, cooperating with GF14e, promotes OsFD1 action on JAZ gene expression. The functional confirmation of Hd3a and OsFD1 in rice defense makes them to be promising targets in molecular rice breeding.

Keywords: Disease resistance; Hd3a; Jasmonic acid pathway; OsFD1; Rice; Xanthomonas oryzae.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cyclopentanes / metabolism
  • Disease Resistance* / drug effects
  • Flagellin / pharmacology
  • Gene Expression Regulation, Plant / drug effects
  • Mutation / genetics
  • Oryza / genetics
  • Oryza / metabolism*
  • Oryza / microbiology*
  • Oxylipins / metabolism
  • Plant Diseases / microbiology*
  • Plant Proteins / genetics
  • Plant Proteins / metabolism*
  • Xanthomonas / drug effects
  • Xanthomonas / physiology*

Substances

  • Cyclopentanes
  • Oxylipins
  • Plant Proteins
  • Flagellin
  • jasmonic acid