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. 2019 May 17;39(5):BSR20190602.
doi: 10.1042/BSR20190602. Print 2019 May 31.

SOX21-AS1 is associated with clinical stage and regulates cell proliferation in nephroblastoma

Jingxiu Zhang  1 , Tianzhao Hou  2 , Xueliang Qi  1 , Jihong Wang  1 , Xiangguo Sun  1
Affiliations

SOX21-AS1 is associated with clinical stage and regulates cell proliferation in nephroblastoma

Jingxiu Zhang et al. Biosci Rep. .

Abstract

LncRNA SOX21 antisense RNA 1 (SOX21-AS1) dysregulated in many types of human cancer, and functioned as tumor suppressor or promoter depending on tumor types. However, there was no report about the role of SOX21-AS1 in nephroblastoma. In the present study, we first found that SOX21-AS1 expression was elevated in nephroblastoma tissues and cell lines compared with adjacent normal tissues and normal human embryonic kidney cell line, respectively. Moreover, we observed nephroblastoma patients with large tumor size, advanced National Wilms Tumor Study (NWTS) stage or unfavorable histopathological type, and patients that had higher SOX21-AS1 expression levels than nephroblastoma patients with small tumor size, early NWTS stage or favorable histopathological type. The in vitro studies suggested that knockdown of SOX21-AS1 suppressed nephroblastoma cell proliferation and colony formation, and induced cell-cycle arrest through up-regulating p57 expression. In conclusion, our study suggests that SOX21-AS1 functions as oncogenic lncRNA in nephroblastoma, which may provide a novel insight for nephroblastoma carcinogenesis.

Keywords: SOX21-AS1; Wilms tumor; biomarker; lncRNA; nephroblastoma.

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Conflict of interest statement

The authors declare that there are no competing interests associated with the manuscript.

Figures

Figure 1
Figure 1. The expression of SOX21-AS1 is up-regulated in nephroblastoma
(A) SOX21-AS1 expression was remarkably increased in nephroblastoma tissues (n=40) compared with corresponding adjacent normal tissues (n=40). (B) SOX21-AS1 expression in nephroblastoma cell lines was higher than normal human embryonic kidney cell line.
Figure 2
Figure 2. The association between SOX21-AS1 expression and clinicopathological features in nephroblastoma patients
(A) Nephroblastoma patients with III-IV stage (n=28) had higher SOX21-AS1 expression levels than nephroblastoma patients with I-II stage (n=12). (B) Nephroblastoma patients with ≥5 cm (n=21) had higher SOX21-AS1 expression levels than nephroblastoma patients with <5 cm (n=19). (C) Nephroblastoma patients with unfavorable histopathological type (n=22) had higher SOX21-AS1 expression levels than nephroblastoma patients with favorable histopathological type (n=18).
Figure 3
Figure 3. The biological function of SOX21-AS1 in nephroblastoma cells
(A) The efficient silencing of SOX21-AS1 in nephroblastoma cells was confirmed by using qRT-PCR. (B) Knockdown of SOX21-AS1 suppressed nephroblastoma cell proliferation. (C) Knockdown of SOX21-AS1 expression inhibited the number of colony formation in nephroblastoma cells. (D) Knockdown of SOX21-AS1 expression induced the cycle arrest at G1/G0 phase in nephroblastoma cells. (*, P<0.01; **, P<0.001).
Figure 4
Figure 4. The molecular mechanism of SOX21-AS1 in nephroblastoma cells
(A) The mRNA levels of p15, p16, p21, p27 and p57 were determined using qRT-PCR following silencing SOX21-AS1 expression in nephroblastoma cells. (B) Knockdown of SOX21-AS1 expression increased p57 protein expression in nephroblastoma cells. (*, P<0.01).
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