Demethylation and desulfonation of textile industry dye, Thiazole Yellow G by Aspergillus niger LAG

Paul O Bankole; Adedotun A Adekunle; Sanjay P Govindwar

doi:10.1016/j.btre.2019.e00327

Demethylation and desulfonation of textile industry dye, Thiazole Yellow G by Aspergillus niger LAG

Biotechnol Rep (Amst). 2019 Mar 28:23:e00327. doi: 10.1016/j.btre.2019.e00327. eCollection 2019 Sep.

Authors

Paul O Bankole^{1

2}, Adedotun A Adekunle³, Sanjay P Govindwar⁴

Affiliations

¹ Department of Pure and Applied Botany, College of Biosciences, Federal University of Agriculture Abeokuta, Ogun State, Nigeria.
² Lancaster Environment Centre, Lancaster University LA1 4YQ, Lancaster, United Kingdom.
³ Department of Botany, University of Lagos, Lagos State, Nigeria.
⁴ Department of Earth Resources and Environmental Engineering, Hanyang University, Seoul, 04763, South Korea.

Abstract

Filamentous fungi perform tremendously in adsorption of dyes from polluted environment. In this study, Aspergillus niger LAG decolorized thiazole yellow G dye within 5 days. Scale up studies done revealed that maximum decolorization (98%) was achieved at a concentration (10 mg L^-1), temperature (35 °C) and pH 6. The fungus exhibited significant inductions in laccase (71%) and lignin peroxidase (48%) respectively. Spectrometric analysis (UV-vis, HPLC and gas chromatography-mass spectrometry) was used in analyzing the degraded products of the dye. The GCMS analysis revealed the production of two metabolites; sodium 6-methyl-2-phenyl-1,3-benzothiazole-7-sulfonate and 2-phenyl-4,5-dihydro-1,3-thiazole after degradation of thiazole yellow G dye. A metabolic pathway of thiazole yellow G dye degradation by Aspergillus niger was proposed. Significant growth in plumule and radicle couple with an attendant increase in germination further confirmed the detoxified status of the dye after degradation.

Keywords: Aspergillus niger; Biodegradation; Detoxification; Thiazole yellow G dye.