A Hyperconversion Mechanism Generates the Chicken Light Chain Preimmune Repertoire

Cell. 1987 Feb 13;48(3):379-88. doi: 10.1016/0092-8674(87)90189-9.

Abstract

The chicken immunoglobulin light chain repertoire has been shown to be entirely derived from a single V lambda 1-J rearranged combination. The complete coding information of the lambda locus was determined: it comprises 25 V-hybridizing elements, all of which are pseudogenes, clustered in both orientations within 19 kb of DNA, starting 2.4 kb upstream of the V lambda 1 gene. Sequences of somatically rearranged V lambda 1 genes from embryonic and posthatching bursal cells show that diversification of light chain sequences occurs during ontogeny by a segmental gene conversion mechanism which takes place at a frequency of 0.05-0.1 per cell generation between the pseudogene pool and the unique rearranged functional V gene.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibody Diversity*
  • Base Sequence
  • Bursa of Fabricius / cytology
  • Chick Embryo
  • Chickens / genetics*
  • Chickens / immunology
  • Gene Conversion*
  • Genetic Variation
  • Immunoglobulin J-Chains / genetics
  • Immunoglobulin Variable Region / genetics*
  • Immunoglobulin lambda-Chains / genetics*

Substances

  • Immunoglobulin J-Chains
  • Immunoglobulin Variable Region
  • Immunoglobulin lambda-Chains

Associated data

  • GENBANK/M15095
  • GENBANK/M15096
  • GENBANK/M15097
  • GENBANK/M15098
  • GENBANK/M15099
  • GENBANK/M15137
  • GENBANK/M15138
  • GENBANK/M15139
  • GENBANK/M15140
  • GENBANK/M15141
  • GENBANK/M15142
  • GENBANK/M15143
  • GENBANK/M15144
  • GENBANK/M15145
  • GENBANK/M15146
  • GENBANK/M15147
  • GENBANK/M15148
  • GENBANK/M15149
  • GENBANK/M15150
  • GENBANK/M15151
  • GENBANK/M15152
  • GENBANK/M15153
  • GENBANK/M15154
  • GENBANK/M15155
  • GENBANK/M15156
  • GENBANK/M15352
  • GENBANK/M21072
  • GENBANK/M21073
  • GENBANK/M21074
  • GENBANK/M21075