ZFP30 promotes adipogenesis through the KAP1-mediated activation of a retrotransposon-derived Pparg2 enhancer

Nat Commun. 2019 Apr 18;10(1):1809. doi: 10.1038/s41467-019-09803-9.


Krüppel-associated box zinc finger proteins (KZFPs) constitute the largest family of mammalian transcription factors, but most remain completely uncharacterized. While initially proposed to primarily repress transposable elements, recent reports have revealed that KFZPs contribute to a wide variety of other biological processes. Using murine and human in vitro and in vivo models, we demonstrate here that one poorly studied KZFP, ZFP30, promotes adipogenesis by directly targeting and activating a retrotransposon-derived Pparg2 enhancer. Through mechanistic studies, we further show that ZFP30 recruits the co-regulator KRAB-associated protein 1 (KAP1), which, surprisingly, acts as a ZFP30 co-activator in this adipogenic context. Our findings provide an understanding of both adipogenic and KZFP-KAP1 complex-mediated gene regulation, showing that the KZFP-KAP1 axis can also function in a non-repressive manner.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3 Cells
  • Adipocytes / physiology
  • Adipogenesis / genetics*
  • Animals
  • Computational Biology
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism*
  • Enhancer Elements, Genetic
  • Female
  • Gene Expression Regulation / physiology
  • Gene Knockout Techniques
  • HEK293 Cells
  • Humans
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • PPAR gamma / genetics
  • Promoter Regions, Genetic / genetics
  • Retroelements / genetics
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*
  • Tripartite Motif-Containing Protein 28 / metabolism*
  • Zinc Fingers / physiology*


  • DNA-Binding Proteins
  • PPAR gamma
  • PPARG protein, human
  • Retroelements
  • Transcription Factors
  • ZFP30 protein, human
  • Zfp30 protein, mouse
  • TRIM28 protein, human
  • Trim28 protein, mouse
  • Tripartite Motif-Containing Protein 28