Elucidating the Host Interactome of EV-A71 2C Reveals Viral Dependency Factors

Ye Li; Xia Jian; Peiqi Yin; Guofeng Zhu; Leiliang Zhang

doi:10.3389/fmicb.2019.00636

Elucidating the Host Interactome of EV-A71 2C Reveals Viral Dependency Factors

Front Microbiol. 2019 Apr 2:10:636. doi: 10.3389/fmicb.2019.00636. eCollection 2019.

Authors

Ye Li¹, Xia Jian¹, Peiqi Yin¹, Guofeng Zhu², Leiliang Zhang³

Affiliations

¹ NHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, China.
² National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, and Key Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.
³ Institute of Basic Medicine, Shandong Academy of Medical Sciences, Jinan, China.

Abstract

Viral protein 2C plays a critical role in EV-A71 replication. The discovery of 2C binding proteins will likely provide potential targets to treat EV-A71 infection. Here, we provide a global proteomic analysis of the human proteins that interact with the EV-A71 2C protein. TRIM4, exportin2, and ARFGAP1 were validated as 2C binding partners. Further functional studies revealed that TRIM4, exportin2, and ARFGAP1 were novel host dependency factors for EV-A71. Moreover, enteroviruses' 2C family proteins interacted with exportin2 and ARFGAP1. In conclusion, our study provides a cellular interactome of the EV-A71 2C and identifies the proviral roles of TRIM4, exportin2, and ARFGAP1 in EV-A71 infection.

Keywords: 2C; ARFGAP1; EV-A71; TRIM4; exportin2.