The azurin gene from Pseudomonas aeruginosa codes for a pre-protein with a signal peptide. Cloning and sequencing of the azurin gene

FEBS Lett. 1987 Feb 9;212(1):168-72. doi: 10.1016/0014-5793(87)81579-x.

Abstract

The azurin gene from Pseudomonas aeruginosa is located on a 1.3 kb long PstI DNA fragment. Its nucleotide sequence has been determined. It appears that the gene codes for a pre-protein with a 19 amino acid long signal sequence which possibly assists in the transport of the azurin over the periplasmic membrane.

MeSH terms

  • Amino Acid Sequence
  • Azurin / genetics*
  • Bacterial Proteins / genetics*
  • Base Sequence
  • Cloning, Molecular
  • Genes, Bacterial*
  • Nucleic Acid Hybridization
  • Protein Precursors / genetics*
  • Protein Sorting Signals / genetics*
  • Pseudomonas aeruginosa / genetics*

Substances

  • Bacterial Proteins
  • Protein Precursors
  • Protein Sorting Signals
  • Azurin