Kallikrein-related peptidase 7(KLK7) is a tumor-related gene. In this study, the full-length coding sequence of porcine KLK7 gene was cloned through RT-PCR. Sequence analysis revealed that the pig KLK7 gene encodes a protein of 257 amino acids which has high homology with the KLK7 protein of six species: polar bear (95%), Weddell seal(94%), dog (92%), Pacific walrus (95%), domestic cat (92%), and Amur tiger (91%). This gene is structured in five exons and four introns as revealed by computer-assisted analysis. Phylogenetic analysis showed that the pig KLK7 gene has a closer genetic relationship with the KLK7 gene of a domestic cat. PCR-Alu I-RFLP was established to detect the GU373714:c.390 C > T substitution of porcine KLK7 gene and eight pig breeds displayed obvious genotype and allele frequency differences at this mutation locus. Association of this SNP with litter size trait was assessed in Large White (n = 200) and Landrace (n = 200) pig populations and results demonstrated that this polymorphic locus was significantly associated with the litter size of all parities in Large White and Landrace sows (p < 0.05). Therefore, KLK7 is also an important reproduction related gene.
Keywords: KLK7; Pig; clone; polymorphism; reproduction.