Receptors for B-cell stimulatory factor-1 expressed on cells of haematopoietic lineage

Nature. 1987 Feb 5-11;325(6104):537-40. doi: 10.1038/325537a0.

Abstract

B-cell stimulatory factor-1 (BSF-1) is a T-cell product of relative molecular mass 20,000 (Mr, 20K) initially described as a cofactor required for DNA synthesis by resting mouse B cells stimulated with low concentrations of anti-IgM antibodies. It acts on resting B cells to enhance the expression of class II major histocompatibility complex (MHC) molecules, to prepare these cells to respond more promptly to subsequent stimuli, such as anti-IgM antibodies, and causes the secretion of IgG1 and IgE by B cells stimulated with lipopolysaccharide (LPS). BSF-1 has been shown to stimulate T cell lines, resting T cells and some mast cell lines. Recently, the designation interleukin-4 (IL-4) has been suggested for BSF-1. We report here the existence of high-affinity cell-surface receptors specific for BSF-1 on both B and T lymphocytes, and on cells of several other haematopoietic lineages, including mast cell, macrophage and undifferentiated haematopoietic cell lines. Resting B and T lymphocytes express receptors, which increase in number upon activation of B cells with LPS or anti-IgM, and of T cells with concanavalin A. Cross-linking of 125I-labelled-BSF-1 to its receptors creates a complex of Mr approximately 80,000.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • B-Lymphocytes / analysis
  • Cell Line
  • Growth Substances / metabolism*
  • Humans
  • Interleukin-4
  • Leukocytes / analysis*
  • Lymphokines / metabolism*
  • Macrophages / analysis
  • Mast Cells / analysis
  • Mice
  • Mice, Inbred DBA
  • Receptors, Immunologic / analysis
  • Receptors, Interleukin-2
  • Receptors, Interleukin-4
  • Receptors, Mitogen / analysis*
  • Spleen / analysis
  • T-Lymphocytes / analysis

Substances

  • Growth Substances
  • Lymphokines
  • Receptors, Immunologic
  • Receptors, Interleukin-2
  • Receptors, Interleukin-4
  • Receptors, Mitogen
  • Interleukin-4