Effects of di-n-butyl phthalate on photosynthetic performance and oxidative damage in different growth stages of wheat in cinnamon soils

Environ Pollut. 2019 Jul;250:357-365. doi: 10.1016/j.envpol.2019.04.022. Epub 2019 Apr 11.

Abstract

Herein, we investigated the effects of di-n-butyl phthalate (DBP) on photosynthesis, ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) content, oxidative damage, and biomass accumulation of different tissues in wheat (Triticum aestivum L) planted in cinnamon soils. The photosynthetic or fluorescence parameters (except for the intercellular carbon dioxide concentration), chlorophyll content, RuBisCO content, and biomass of roots, stems, and leaves decreased at the seedling, jointing, and booting stages under the stress of DBP. Compared with the control, the content of superoxide anions and hydrogen peroxide in the roots, stems, and leaves increased with increasing DBP concentrations at the seedling, jointing, and booting stages. The activities of superoxide dismutase (SOD) and catalase (CAT) in the roots, stems, and leaves increased under the 10 and 20 mg kg-1 DBP treatments; however, no significant changes were observed under the 40 mg kg-1 DBP treatment at the seedling stage (except for the SOD activity in roots). The increase in SOD and CAT activities in the roots, stems, and leaves with increasing DBP concentration at the jointing and booting stages suggested that an increase in the activities of these antioxidant enzymes may play an important role in defending against excess reactive oxygen species under DBP stress. The biomass of wheat roots, stems, and leaves decreased with an increase in DBP concentration, which was presumably caused by a decrease in photosynthesis and RuBisCO. The effect of DBP on wheat roots, stems, and leaves decreased with wheat growth.

Keywords: Biomass; Di-n-butyl phthalate; Oxidative damage; RuBisCO; Wheat.

MeSH terms

  • Antioxidants / metabolism
  • Catalase / metabolism
  • Chlorophyll / metabolism
  • Cinnamomum zeylanicum
  • Dibutyl Phthalate / toxicity*
  • Hydrogen Peroxide / pharmacology
  • Oxidation-Reduction
  • Oxidative Stress / physiology
  • Photosynthesis / drug effects*
  • Plant Leaves / chemistry
  • Plant Roots / metabolism
  • Reactive Oxygen Species
  • Ribulose-Bisphosphate Carboxylase / metabolism
  • Seedlings / metabolism
  • Soil / chemistry
  • Soil Pollutants / analysis
  • Soil Pollutants / toxicity*
  • Superoxide Dismutase / metabolism
  • Triticum / drug effects
  • Triticum / metabolism
  • Triticum / physiology*

Substances

  • Antioxidants
  • Reactive Oxygen Species
  • Soil
  • Soil Pollutants
  • Chlorophyll
  • Dibutyl Phthalate
  • Hydrogen Peroxide
  • Catalase
  • Superoxide Dismutase
  • Ribulose-Bisphosphate Carboxylase