Jasmonate-mediated wound signalling promotes plant regeneration

Guifang Zhang; Fei Zhao; Lyuqin Chen; Yu Pan; Lijun Sun; Ning Bao; Teng Zhang; Chun-Xiao Cui; Zaozao Qiu; Yijing Zhang; Li Yang; Lin Xu

doi:10.1038/s41477-019-0408-x

Jasmonate-mediated wound signalling promotes plant regeneration

Nat Plants. 2019 May;5(5):491-497. doi: 10.1038/s41477-019-0408-x. Epub 2019 Apr 22.

Authors

Guifang Zhang^#^{1

2}, Fei Zhao^#^{1

2}, Lyuqin Chen^#^{1

2

3

4}, Yu Pan⁵, Lijun Sun⁵, Ning Bao⁶, Teng Zhang^{1

2}, Chun-Xiao Cui^{2

7}, Zaozao Qiu⁷, Yijing Zhang¹, Li Yang⁸, Lin Xu⁹

Affiliations

¹ National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, China.
² University of Chinese Academy of Sciences, Beijing, China.
³ Department of Neurosurgery, Stanford University School of Medicine, Stanford, CA, USA.
⁴ Department of Psychiatry and Behavioral Sciences, Stanford University School of Medicine, Stanford, CA, USA.
⁵ School of Life Sciences, Nantong University, Nantong, China.
⁶ School of Public Health, Nantong University, Nantong, China.
⁷ Shanghai-Hong Kong Joint Laboratory in Chemical Synthesis, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai, China.
⁸ Department of Plant Pathology, University of Georgia, Athens, GA, USA.
⁹ National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, China. xulin01@sibs.ac.cn.

^# Contributed equally.

PMID: 31011153
DOI: 10.1038/s41477-019-0408-x

Abstract

Wounding is the first event triggering regeneration^1-4. However, the molecular basis of wound signalling pathways in plant regeneration is largely unclear. We previously established a method to study de novo root regeneration (DNRR) in Arabidopsis thaliana^5,6, which provides a platform for analysing wounding. During DNRR, auxin is biosynthesized after leaf detachment and promotes cell fate transition to form the root primordium^5-7. Here, we show that jasmonates (JAs) serve as a wound signal during DNRR. Within 2 h of leaf detachment, JA is produced in leaf explants and activates ETHYLENE RESPONSE FACTOR109 (ERF109). ERF109 upregulates ANTHRANILATE SYNTHASE α1 (ASA1)-a tryptophan biosynthesis gene in the auxin production pathway^8-10-dependent on the pre-deposition of SET DOMAIN GROUP8 (SDG8)-mediated histone H3 lysine 36 trimethylation (H3K36me3)¹¹ on the ASA1 locus. After 2 h, ERF109 activity is inhibited by direct interaction with JASMONATE-ZIM-DOMAIN (JAZ) proteins to prevent hypersensitivity to wounding. Our results suggest that a dynamic JA wave cooperates with histone methylation to upregulate a pulse of auxin production and promote DNRR in response to wounding.

Publication types

Letter
Research Support, Non-U.S. Gov't

MeSH terms

Arabidopsis / physiology*
Cyclopentanes / metabolism*
Oxylipins / metabolism*
Plant Growth Regulators / metabolism*
Plant Growth Regulators / physiology
Plant Roots / physiology*
Real-Time Polymerase Chain Reaction
Regeneration* / physiology
Signal Transduction* / physiology
Two-Hybrid System Techniques

Substances

Cyclopentanes
Oxylipins
Plant Growth Regulators
jasmonic acid