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. 2019 Apr 13;15:36.
doi: 10.1186/s13007-019-0421-0. eCollection 2019.

In Vitro Propagation Method for Production of Morphologically and Genetically Stable Plants of Different Strawberry Cultivars

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Free PMC article

In Vitro Propagation Method for Production of Morphologically and Genetically Stable Plants of Different Strawberry Cultivars

Aung Htay Naing et al. Plant Methods. .
Free PMC article

Abstract

Background: As strawberries are susceptible to somaclonal variation when propagated by tissue culture techniques, it is challenging to obtain the true-to-type plants necessary for continuous production of fruits of stable quality. Therefore, we aimed to develop an in vitro propagation method for the production of true-to-type plants of five different strawberry cultivars from meristems cultured in media containing different concentrations of kinetin (Kn).

Results: For all the cultivars, shoot induction was successful only in the meristems cultured in the medium without Kn and the medium containing 0.5 mg L-1 Kn. The shoots obtained from explants cultured in media supplemented with 0.5 mg L-1 Kn exhibited better plant growth parameters than those cultured in media without Kn and were genetically stable when compared with conventionally propagated plants for all the cultivars. Vegetative and sexual characters and fruit quality attributes observed in the plants derived from meristems cultured on 0.5 mg L-1 Kn and the conventionally propagated plants were not significantly different when grown for three continuous growing seasons under greenhouse conditions.

Conclusion: The culture of meristems in the medium containing 0.5 mg L-1 Kn is suitable for the efficient propagation of true-to-type plants of different strawberry cultivars and continuous production of fruits with stable quality. Hence, we expect that the method presented in this study will be helpful for the commercial production of true-to-type plants generated in vitro for other strawberry cultivars.

Keywords: Field performance; Fruit quality; Genetic variation; Kinetin; Meristem.

Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Fig. 1
Fig. 1
Mother plants grown in the greenhouse (a) and runner tips with one leaf primordia (b) used as plant materials. Arrows indicate apical meristems
Fig. 2
Fig. 2
In vitro regeneration of shoots from meristems: a runner tip used as explant source; b culture of the meristem excised from the runner tips on the medium containing Kn 0.5 mg L−1; c induction of shoots from the meristem after 8 weeks of culture; d rooting of the meristem-derived plants on the rooting media containing indole-3-butyric acid after 6 weeks of culture. Size bars for figures (ad) indicate 1.0, 0.5, 1.0, and 1.0 cm, respectively
Fig. 3
Fig. 3
Effect of kinetin on in vitro regeneration of shoots from the meristem of different strawberry cultivars after 14 weeks of culture: a number of shoots; b number of leaves; c number of roots per shoot. Data represent the mean of three replicates, and the bar indicates the standard deviation. Means with asterisk(s) are statistically significant (t-test, *P < 0.05)
Fig. 4
Fig. 4
Number of surviving plants per explant of different strawberry cultivars after acclimatization. Data represent the mean of three replicates, and the bar indicates the standard deviation. Means with asterisk(s) are statistically significant (t-test, *P < 0.05)
Fig. 5
Fig. 5
Meristem-derived plants (obtained from explants cultured on medium supplemented with 0.5 mg L−1 kinetin) of different strawberry cultivars cultivated in the greenhouse
Fig. 6
Fig. 6
Comparison of the ploidy levels between meristem-derived plants (Kinetin) and conventionally propagated plants (control) of different strawberry cultivars: a Santa; b Fanta; c Berrystar; d Honeybell; e Okhyang
Fig. 7
Fig. 7
Detection of somaclonal variation between meristem-derived plants (Kinetin) and conventionally propagated plants (control) of different strawberry cultivars using RAPD markers: a Santa, b Fanta, c Berrystar, d Honeybell, e Okhyang
Fig. 8
Fig. 8
Comparison of vegetative and sexual traits between meristem-derived plants (Kin) and conventionally propagated plants (Cont) of different strawberry cultivars: a canopy size; b number of leaves; c number of flowers; d number of shoots. Data represent the mean of three growing seasons, and the bar indicates the standard deviation. Means with asterisk(s) are statistically significant (t-test, *P < 0.05)
Fig. 9
Fig. 9
Comparison of fruit characters between meristem-derived plants (Kin) and conventionally propagated plants (Cont) of different strawberry cultivars: a fruit fresh weight; b fruit length; c fruit width. Data represent the mean of three growing seasons, and the bar indicates the standard deviation. Means with asterisk(s) are statistically significant (t-test, *P < 0.05)
Fig. 10
Fig. 10
Comparison of total sugar content (a) and firmness (b) per fruit between meristem-derived plants (Kin) and conventionally propagated plants (Cont) of different strawberry cultivars. Data represent the mean of three growing seasons, and the bar indicates the standard deviation. Means with asterisk(s) are statistically significant (t-test, *P < 0.05)

Cited by 1 article

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