Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1987;92(3-4):233-42.
doi: 10.1007/BF01317480.

Detection of DNA Viruses by Radioactive and Non Radioactive DNA Probes: Application to African Swine Fever Virus

Detection of DNA Viruses by Radioactive and Non Radioactive DNA Probes: Application to African Swine Fever Virus

E Tabarés. Arch Virol. .

Abstract

A molecular hybridization technique using radioactive and non radioactive DNA probes, has been used to detect ASFV DNA immobilized on nitrocellulose paper. It is based on the use of plasmid pRPEL-2 as a hybridization probe. This plasmid contain the H-ClaI DNA fragment (size 5.6 Kbp) from the Spain-70 strain of ASFV. The sensitivity of detection using radioactive 32P-probes (specific activity about 2 X 10(8) cpm per microgram) was about 20 pg of viral DNA. The 32P-pRPEL-2 DNA probe can detect about 100 infected MS cells and failed to hybridize to DNA from HSV-2, MS cells or salmon sperm. The sensitivity with non radioactive probes was about 4 ng of viral DNA for a sulfonated DNA probe and 400 pg for a biotinylated DNA probe. The efficiency of DNA fixation to the filter, the effect of EDTA and of ultrasonic treatment of the sample were also investigated.

Similar articles

See all similar articles

Cited by 8 articles

See all "Cited by" articles

References

    1. J Clin Microbiol. 1984 Mar;19(3):436-8 - PubMed
    1. Arch Virol. 1981;70(3):297-300 - PubMed
    1. Arch Virol. 1986;87(1-2):119-25 - PubMed
    1. Anal Biochem. 1977 Nov;83(1):252-7 - PubMed
    1. J Clin Microbiol. 1985 Dec;22(6):990-5 - PubMed

Publication types

MeSH terms

Feedback