This study evaluated the biocompatibility of degradable polydioxanone (PDS) electrospun drug delivery systems (hereafter referred as matrices) containing metronidazole (MET) or ciprofloxacin (CIP) after subcutaneous implantation in rats. Sixty adult male rats were randomized into six groups: SHAM (sham surgery); PDS (antibiotic-free matrix); 1MET (one 25 wt% MET matrix); 1CIP (one 25 wt% CIP matrix); 2MET (two 25 wt% MET matrices); and 2CIP (two 25 wt% CIP matrices). At 3 and 30 days, animals were assessed for inflammatory cell response (ICR), collagen fibers degradation, and oxidative profile (reactive oxygen species [ROS]; lipid peroxidation [LP]; and protein carbonyl [PC]). At 3 days, percentages of no/discrete ICR were 100, 93.3, 86.7, 76.7, 50, and 66.6 for SHAM, PDS, 1MET, 1CIP, 2MET, and 2CIP, respectively. At 30 days, percentages of no/discrete ICR were 100% for SHAM, PDS, 1MET, and 1CIP and 93.3% for 2MET and 2CIP. Between 3 and 30 days, SHAM, 1CIP, and 2CIP produced collagen, while 1MET and 2MET were unchanged. At 30 days, the collagen fiber means percentages for SHAM, PDS, 1MET, 1CIP, 2MET, and 2CIP were 63.7, 60.7, 56.6, 62.6, 51.8, and 61.7, respectively. Antibiotic-eluting matrices showed similar or better oxidative behavior when compared to PDS, except for CIP-eluting matrices, which showed higher levels of PC compared to SHAM or PDS at 30 days. Collectively, our findings indicate that antibiotic-eluting matrices may be an attractive biocompatible drug delivery system to fight periodontopathogens. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B, 2019.
Keywords: biomaterials; drug delivery; electrospinning; nanofibers; periodontitis.
© 2019 Wiley Periodicals, Inc.
Biodegradable Nanofibrous Drug Delivery Systems: Effects of Metronidazole and Ciprofloxacin on Periodontopathogens and Commensal Oral BacteriaMC Bottino et al. Clin Oral Investig 18 (9), 2151-8. PMID 24535074.CIP-containing matrices led to a significant inhibition of periodontopathogens without negatively impairing the growth of periodontal beneficial bacteria.
Effects of Ciprofloxacin-Containing Scaffolds on Enterococcus Faecalis BiofilmsMT Albuquerque et al. J Endod 41 (5), 710-4. PMID 25698261.On the basis of the data presented, newly developed antibiotic-containing electrospun scaffolds hold promise as an intracanal medicament to eliminate biofilm/infection be …
Bioactive Nanofibrous Scaffolds for Regenerative EndodonticsMC Bottino et al. J Dent Res 92 (11), 963-9. PMID 24056225.Here we report the synthesis, materials characterization, antimicrobial capacity, and cytocompatibility of novel antibiotic-containing scaffolds. Metronidazole (MET) or C …
Dental Pulp Stem Cell Responses to Novel Antibiotic-Containing Scaffolds for Regenerative EndodonticsK Kamocki et al. Int Endod J 48 (12), 1147-56. PMID 25425048.Synthesized antibiotic-containing scaffolds had significantly lower effects on hDPSCs proliferation when compared to the saturated CIP/MET solution (DAP).
Drug Loading and Release From Electrospun Biodegradable NanofibersN Goonoo et al. J Biomed Nanotechnol 10 (9), 2173-99. PMID 25992453. - ReviewThis review explores the potential of electrospun nanofibers for drug delivery applications. In the first section, some of the key challenges in drug delivery as well as …