Evaluation of some biological, biochemical, and hematological aspects in male albino rats after acute exposure to the nano-structured oxides of nickel and cobalt

Environ Sci Pollut Res Int. 2019 Jun;26(17):17407-17417. doi: 10.1007/s11356-019-05093-2. Epub 2019 Apr 24.


Nanomaterial applications are a fast-developing field. In spite of their powerful advantages, many open questions regarding how these small-sized chemicals may influence the environment and human health. However, scarce reports are available on the potential hazards of combined nanoparticles, taken into consideration that nickel oxide (NiO) and cobalt (II, III) oxide (Co3O4) nanoparticles (NPs) are already used together in many applications. Hence, the present work was designed to study the probable changes in some biological, hematological, and serum biochemical variables throughout 2 weeks following an oral administration of 0.5 g and 1.0 g of NiO-NPs or/and Co3O4-NPs per kilogram body weight of rats. As compared with the controls, the exposure to NiO-NPs or Co3O4-NPs solely caused significant elevations in the relative weights of brain (RBW), kidney (RKW) and liver (RLW), water consumption (WC), red blood cells (RBCs) count, hemoglobin (Hb) content, packed cell volume (PCV), and serum levels of low-density lipoprotein cholesterol (LDL-C), glucose, creatinine, urea, and uric acid as well as serum activities of aspartate and alanine aminotransferases (ASAT and ALAT). In addition, remarkable declines in the total body weight (TBW), feed consumption (FC), white blood cells (WBCs) count, serum levels of total protein (TP), albumin, albumin/globulin ratio, total cholesterol (TC), triglycerides (TG), and high-density lipoprotein cholesterol (HDL-C) were caused by administration of NiO-NPs or Co3O4-NPs, separately. On contrary, the co-administration of NiO-NPs and Co3O4-NPs together caused less noticeable changes in most of studied variables as compared with those administered NiO-NPs or Co3O4-NPs, individually. In conclusion, the exposure to a combination of NiO-NPs and Co3O4-NPs suppressed the adverse effects of the individual NPs on the studied variables.

Keywords: Biological variables; Cobalt oxide; Hematology; Metabolism; Nanoparticles; Nickel oxide.

MeSH terms

  • Animals
  • Cobalt / toxicity*
  • Humans
  • Kidney / drug effects
  • Liver / drug effects
  • Male
  • Metal Nanoparticles / toxicity*
  • Nickel / toxicity*
  • Oxides / metabolism
  • Rats
  • Toxicity Tests


  • Oxides
  • Cobalt
  • Nickel
  • nickel monoxide