NMR for sample quality assessment in metabolomics

Veronica Ghini; Deborah Quaglio; Claudio Luchinat; Paola Turano

doi:10.1016/j.nbt.2019.04.004

NMR for sample quality assessment in metabolomics

N Biotechnol. 2019 Sep 25:52:25-34. doi: 10.1016/j.nbt.2019.04.004. Epub 2019 Apr 22.

Authors

Veronica Ghini¹, Deborah Quaglio², Claudio Luchinat³, Paola Turano⁴

Affiliations

¹ Center of Magnetic Resonance (CERM), University of Florence, Sesto Fiorentino FI, Italy.
² Department of Chemistry and Technology of Drugs, Sapienza University of Rome, Rome, Italy.
³ Center of Magnetic Resonance (CERM), University of Florence, Sesto Fiorentino FI, Italy; Department of Chemistry, University of Florence, Sesto Fiorentino FI, Italy.
⁴ Center of Magnetic Resonance (CERM), University of Florence, Sesto Fiorentino FI, Italy; Department of Chemistry, University of Florence, Sesto Fiorentino FI, Italy. Electronic address: turano@cerm.unifi.it.

PMID: 31022482
DOI: 10.1016/j.nbt.2019.04.004

Abstract

The EU Framework 7 project SPIDIA was the occasion for development of NMR approaches to evaluate the impact of different pre-analytical treatments on the quality of biological samples dedicated to metabolomics. Systematic simulation of different pre-analytical procedures was performed on urine and blood serum and plasma. Here we review the key aspects of these studies that have led to the development of CEN technical specifications, to be translated into ISO/IS in the course of the EU Horizon 2020 project SPIDIA4P. Inspired by the SPIDIA results, follow-up research was performed, extending the analysis to different sample types and to the different effects of long-term storage. The latter activity was in conjunction with the local European da Vinci Biobank. These results (which partially contributed to the ANNEX of CEN/TS 16945"MOLECULAR IN VITRO DIAGNOSTIC EXAMINATIONS - SPECIFICATIONS FOR PRE-EXAMINATION PROCESSES FOR METABOLOMICS IN URINE, VENOUS BLOOD SERUM AND PLASMA") are presented in detail.

Keywords: Plasma; Pre-analytics; Serum; Urine.

MeSH terms

Humans
Magnetic Resonance Spectroscopy*
Metabolomics*
Quality Control
Serum / chemistry
Time Factors