A line of non-tumorigenic mouse melanocytes, syngeneic with the B16 melanoma and requiring a tumour promoter for growth

Int J Cancer. 1987 Mar 15;39(3):414-8. doi: 10.1002/ijc.2910390324.

Abstract

An immortal line of pigmented melanocytes, "melan-a", has been derived from normal epidermal melanoblasts from embryos of inbred C57BL mice. The conditions favouring proliferation of these cells largely resemble those for normal, non-established mouse melanoblasts and melanocytes, and include a low extracellular pH and the presence of a tumour promoter, tetradecanoyl phorbol acetate (TPA) or teleocidin. Melan-a cells have the diploid chromosome number and do not form tumours in syngeneic or nude mice. They are therefore the first known line of non-tumorigenic mouse melanocytes, although an aneuploid melanocyte line of untested tumorigenicity has been reported (Sato et al., 1985). Melan-a cells are syngeneic with the B16 melanoma and its sublines, and provide an excellent parallel non-tumorigenic line for studies of the cellular and molecular basis of melanoma malignancy.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Division
  • Cell Line*
  • Hydrogen-Ion Concentration
  • Lyngbya Toxins / pharmacology
  • Melanocytes / physiology*
  • Melanoma / pathology
  • Mice
  • Mice, Inbred BALB C
  • Tetradecanoylphorbol Acetate / pharmacology*

Substances

  • Lyngbya Toxins
  • teleocidins
  • Tetradecanoylphorbol Acetate