malM, a New Gene of the Maltose Regulon in Escherichia Coli K12. II. Mutations Affecting the Signal Peptide of the MalM Protein

J Mol Biol. 1986 Oct 5;191(3):313-20. doi: 10.1016/0022-2836(86)90128-2.


malM is the last gene of the malK-lamB-malM operon of Escherichia coli K12. It encodes a periplasmic protein. Mutations affecting the hydrophobic core of the N-terminal extension of the MalM protein have been isolated. They result in an increase in amount and specific activity of a MalM-LacZ hybrid protein. This result confirms that the signal peptide of the MalM protein is functional.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • DNA, Bacterial
  • Escherichia coli / enzymology
  • Escherichia coli / genetics*
  • Genes, Bacterial*
  • Maltose / genetics*
  • Mutation*
  • Phenotype
  • Protein Biosynthesis
  • Protein Sorting Signals / genetics*
  • Terminator Regions, Genetic
  • beta-Galactosidase / metabolism


  • DNA, Bacterial
  • Protein Sorting Signals
  • Maltose
  • beta-Galactosidase