Zinc Sulfate in Narrow Range as an In Vitro Anti-HSV-1 Assay

doi:10.1007/s12011-019-01728-0

. 2020 Feb;193(2):410-413.

doi: 10.1007/s12011-019-01728-0. Epub 2019 Apr 26.

Zinc Sulfate in Narrow Range as an In Vitro Anti-HSV-1 Assay

Mona Fani¹, Nastaran Khodadad¹, Saeedeh Ebrahimi¹, Rahil Nahidsamiei¹, Manoocher Makvandi¹, Ali Teimoori^{2

3}, Hadis Langari⁴

Affiliations

¹ Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
² Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. teimooriali1982@gmail.com.
³ Department of Virology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran. teimooriali1982@gmail.com.
⁴ Department of Modern Sciences and Technologies, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

PMID: 31028520
PMCID: PMC7090960
DOI: 10.1007/s12011-019-01728-0

Zinc Sulfate in Narrow Range as an In Vitro Anti-HSV-1 Assay

Mona Fani et al. Biol Trace Elem Res. 2020 Feb.

. 2020 Feb;193(2):410-413.

doi: 10.1007/s12011-019-01728-0. Epub 2019 Apr 26.

Authors

Mona Fani¹, Nastaran Khodadad¹, Saeedeh Ebrahimi¹, Rahil Nahidsamiei¹, Manoocher Makvandi¹, Ali Teimoori^{2

3}, Hadis Langari⁴

Affiliations

¹ Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
² Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. teimooriali1982@gmail.com.
³ Department of Virology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran. teimooriali1982@gmail.com.
⁴ Department of Modern Sciences and Technologies, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

PMID: 31028520
PMCID: PMC7090960
DOI: 10.1007/s12011-019-01728-0

Abstract

This report explains the employing of a combination test of traditional cell culture with a quantitative real-time PCR for assessment of the antiviral effect of zinc sulfate (ZnSO₄) on herpes simplex virus (HSV)-infected Vero cells. Our evidence showed that the treatment with 0.3 mM ZnSO₄ strongly inhibited the replication of virus progeny (MOI 0.001) at least 68-fold less. On the other hand, the IC50 demonstrated that the highest activity of ZnSO₄ was at the 0.23 mM concentration.

Keywords: Antiviral effect; HSV; Herpes simplex virus; Zinc sulfate.

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Figures

**Fig. 1**
Microscopic morphology of HSV-1 propagation in Vero cells

**Fig. 2**
Inhibitory effects of various concentrations of ZnSO₄ on HSV-1 (strain KOS)-infected Vero cells at 0.001 (a) and 0.01 MOI (b)

**Fig. 3**
Viability percentage of Vero cells in exposure to increasing concentrations of ZnSO₄

See this image and copyright information in PMC

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References

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[1] Wolfe ND, Dunavan CP, Diamond J. Origins of major human infectious diseases. Nature. 2007;447(7142):279–283. doi: 10.1038/nature05775. - DOI - PMC - PubMed

[2] Wolfe ND, Dunavan CP, Diamond J. Origins of major human infectious diseases. Nature. 2007;447(7142):279–283. doi: 10.1038/nature05775. - DOI - PMC - PubMed

[3] Whitley RJ, Nahmias AJ, Visintine AM, Fleming CL, Alford CA, Yeager A, et al. The natural history of herpes simplex virus infection of mother and newborn. Pediatrics. 1980;66(4):489–494. - PubMed

[4] Whitley RJ, Nahmias AJ, Visintine AM, Fleming CL, Alford CA, Yeager A, et al. The natural history of herpes simplex virus infection of mother and newborn. Pediatrics. 1980;66(4):489–494. - PubMed

[5] Pasternak B, Hviid A. Use of acyclovir, valacyclovir, and famciclovir in the first trimester of pregnancy and the risk of birth defects. JAMA. 2010;304(8):859–866. doi: 10.1001/jama.2010.1206. - DOI - PubMed

[6] Pasternak B, Hviid A. Use of acyclovir, valacyclovir, and famciclovir in the first trimester of pregnancy and the risk of birth defects. JAMA. 2010;304(8):859–866. doi: 10.1001/jama.2010.1206. - DOI - PubMed

[7] Gaby AR. Natural remedies for herpes simplex. Altern Med Rev. 2006;11(2):93. - PubMed

[8] Gaby AR. Natural remedies for herpes simplex. Altern Med Rev. 2006;11(2):93. - PubMed

[9] Jiang Y-C, Feng H, Lin Y-C, Guo X-R. New strategies against drug resistance to herpes simplex virus. Int J Oral Sci. 2016;8(1):1–6. doi: 10.1038/ijos.2016.3. - DOI - PMC - PubMed

[10] Jiang Y-C, Feng H, Lin Y-C, Guo X-R. New strategies against drug resistance to herpes simplex virus. Int J Oral Sci. 2016;8(1):1–6. doi: 10.1038/ijos.2016.3. - DOI - PMC - PubMed

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Zinc Sulfate in Narrow Range as an In Vitro Anti-HSV-1 Assay

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Zinc Sulfate in Narrow Range as an In Vitro Anti-HSV-1 Assay

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