Single-Cell RNA Sequencing with Drop-Seq

Methods Mol Biol. 2019;1979:73-85. doi: 10.1007/978-1-4939-9240-9_6.

Abstract

Drop-Seq is a low-cost, high-throughput platform to profile thousands of cells by encapsualting them into individual droplets. Uniquely barcoded mRNA capture microparticles and cells are coconfined through a microfluidic device within the droplets where they undergo cell lysis and RNA hybridiztion. After breaking the droplets and pooling the hybridized particles, reverse transcription, PCR, and sequencing in single reactions allow to generate data from thousands of single-cell transcriptomes while maintaining information on the cellular origin of each transcript.

Keywords: Cell barcoding; Drop-seq; Droplet technology; Microfluidics; Single-cell RNA-sequencing; Systems biology; Transcriptomics.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, N.I.H., Intramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Equipment Design
  • Gene Expression Profiling / economics
  • Gene Expression Profiling / instrumentation*
  • Gene Expression Profiling / methods
  • Gene Library
  • High-Throughput Nucleotide Sequencing / economics
  • High-Throughput Nucleotide Sequencing / instrumentation*
  • High-Throughput Nucleotide Sequencing / methods
  • Humans
  • Lab-On-A-Chip Devices* / economics
  • Single-Cell Analysis / economics
  • Single-Cell Analysis / instrumentation*
  • Single-Cell Analysis / methods
  • Transcriptome