Sequencing the Genomes of Single Cells

Methods Mol Biol. 2019;1979:227-234. doi: 10.1007/978-1-4939-9240-9_14.


Single-cell genome sequencing can detect low-frequency genetic alterations present in complex tissues. However, the experimental procedures are technically challenging. This includes dissociation of the tissue, isolation of single cells, whole-genome amplification, sequencing library preparation, and an optional target enrichment. Here we describe how to perform each of these processes to obtain high-quality single-cell genome sequencing data.

Keywords: DOP-PCR; Genomics; MDA; Sequencing library preparation; Single-cell isolation; Target enrichment; WGA; Whole-genome amplification (WGA).

MeSH terms

  • Animals
  • Cell Separation / methods*
  • DNA / genetics
  • DNA Copy Number Variations
  • Gene Library
  • Genome
  • Genomics / methods*
  • Humans
  • Nucleic Acid Amplification Techniques / methods
  • Oligonucleotide Array Sequence Analysis / methods*
  • Single-Cell Analysis / methods*


  • DNA