Differential phosphorylation of proteins is a key regulatory mechanism in biology. Immunoprecipitation-coupled mass spectrometry facilitates the targeted analysis of transient receptor ion potential channel polycystin-2 (TRPP2) phosphorylation. However, empirical testing is required to optimize experimental conditions for immunoprecipitation and mass spectrometry. Here, we present a detailed workflow for the reliable analysis of endogenous TRPP2 phosphorylation in differentiated renal epithelial cells.
Keywords: ADPKD; Immunoprecipitation; Mass spectrometry; PKD2; Phosphoproteomics; Phosphorylation; Polycystic kidney disease; Post-translational modification; TRPP2.