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. 2019 Jun;59(2):147-153.
doi: 10.1007/s12088-019-00797-y. Epub 2019 Mar 19.

Antimicrobial Activity of Compounds Containing Silver Nanoparticles and Calcium Glycerophosphate in Combination With Tyrosol

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Free PMC article

Antimicrobial Activity of Compounds Containing Silver Nanoparticles and Calcium Glycerophosphate in Combination With Tyrosol

José Antonio Santos Souza et al. Indian J Microbiol. .
Free PMC article

Abstract

Nanocomposites containing antimicrobial agents and calcium phosphates have been developed. Thus, this study assessed the effects of two compounds containing silver nanoparticles (AgNPs) and β-calcium glycerophosphate (CaGP), associated or not with tyrosol (TYR), against planktonic cells and biofilms of Candida albicans and Streptococcus mutans. The nanocompounds were synthesized through chemical and 'green' processes and characterized by scanning electron microscopy. The minimum and fractional inhibitory concentrations of each compound were determined for planktonic cells. Next, 24-h single biofilms of C. albicans and S. mutans were treated for 24 h with the nanocompounds alone or in combination with TYR, and the antibiofilm effect was assessed through enumeration of colony forming units. Biofilm data were statistically examined using one-way ANOVA and the Kruskal-Wallis test (α = 0.05). The chemically synthesized nanocompound in combination with TYR demonstrated a synergistic effect against planktonic cells of C. albicans and S. mutans. For the nanocompound obtained through the 'green' route associated with TYR, a synergistic effect was observed only against C. albicans. For biofilms, only the combination obtained through the 'green' route + TYR demonstrated a synergistic effect against C. albicans. Our results may contribute to the development of oral care products containing AgNPs-CaGP and TYR to combat oral infections.

Keywords: Biofilms; Candida albicans; Metal nanoparticles; Polyphosphates; Streptococcus mutans.

Figures

Fig. 1
Fig. 1
Scanning electron microscopy images and average size (histograms) of the AgNPs–CaGP nanocompounds: a Compound obtained by the chemical process (C); b Compound obtained by the ‘green’ process (G). Magnification:  30,000 x (1a), and  60,000 x (1b)
Fig. 2
Fig. 2
a Mean values (± SD) of the logarithm of colony forming units per cm2 (log10 CFU/cm2) for single biofilms of C. albicans and S. mutans. NC = negative control; C = nanocompound obtained by the chemical process; TYR = tyrosol. Concentrations tested for C. albicans: C at 4000 µg/mL and TYR at 6908 µg/mL. For S. mutans: C at 1000 µg/mL and TYR at 1727 µg/mL. Different lowercase and uppercase letters indicate significant differences between the treatments for C. albicans (p < 0.05; Kruskal–Wallis test followed by the Tukey test) and S. mutans (p < 0.05; Kruskal–Wallis test followed by the Tukey test) biofilms, respectively. b Mean values (± SD) of the logarithm of colony forming units per cm2 (log10 CFU/cm2) for single biofilms of C. albicans. NC = negative control; G = nanocompound obtained by the ‘green’ route at 7810 µg/mL; TYR = tyrosol at 3454 µg/mL; G + TYR = 7810 µg/mL of G and 3454 µg/mL of TYR. Different lowercase letters indicate significant differences between the treatments (p < 0.05; Kruskal–Wallis test followed by the Student–Newman–Keuls test)

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