A quantitative method for detection of circulating fms related tyrosine kinase 3 (FLT-3) in acute myeloid leukemia (AML) patients

J Immunol Methods. 2019 Jul:470:55-58. doi: 10.1016/j.jim.2019.04.010. Epub 2019 Apr 26.

Abstract

FMS related tyrosine kinase 3 (FLT-3) is a tyrosine kinase expressed in early hematopoietic precursor cells and has roles in survival, proliferation, and differentiation. Bone marrow expression and mutagenic analysis of FLT-3 in Acute Myeloid Leukemia (AML) patients is well-characterized. However, the levels of circulating FLT-3 in serum have not been previously described. In this study we describe a quantitative electrochemiluminescent immunoassay that detects FLT-3 in human serum. Using this method we find that AML patients have elevated levels of circulating FLT-3 and these levels correlated to the percent blast counts in the bone marrow (BM).

Keywords: Acute myeloid leukemia; Biomarker; FMS related tyrosine kinase 3; Immunoassay.

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Biomarkers, Tumor / blood*
  • Biomarkers, Tumor / genetics
  • Bone Marrow / enzymology
  • Bone Marrow / pathology
  • Electrochemical Techniques / methods*
  • Electrochemical Techniques / standards
  • Female
  • Gene Expression
  • Humans
  • Immunoassay / methods*
  • Immunoassay / standards
  • Leukemia, Myeloid, Acute / blood
  • Leukemia, Myeloid, Acute / diagnosis*
  • Leukemia, Myeloid, Acute / genetics
  • Leukemia, Myeloid, Acute / pathology
  • Luminescence
  • Luminescent Measurements / methods*
  • Luminescent Measurements / standards
  • Male
  • Middle Aged
  • Observer Variation
  • Reproducibility of Results
  • Sensitivity and Specificity
  • fms-Like Tyrosine Kinase 3 / blood*
  • fms-Like Tyrosine Kinase 3 / genetics

Substances

  • Biomarkers, Tumor
  • FLT3 protein, human
  • fms-Like Tyrosine Kinase 3