A humanized MDCK cell line for the efficient isolation and propagation of human influenza viruses

Nat Microbiol. 2019 Aug;4(8):1268-1273. doi: 10.1038/s41564-019-0433-6. Epub 2019 Apr 29.

Abstract

Here, we developed hCK, a Madin-Darby canine kidney (MDCK) cell line that expresses high levels of human influenza virus receptors and low levels of avian virus receptors. hCK cells supported human A/H3N2 influenza virus isolation and growth much more effectively than conventional MDCK or human virus receptor-overexpressing (AX4) cells. A/H3N2 viruses propagated in hCK cells also maintained higher genetic stability than those propagated in MDCK and AX4 cells.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, CD / metabolism
  • Cell Line
  • Dogs
  • Humans
  • Influenza A Virus, H3N2 Subtype / genetics
  • Influenza A Virus, H3N2 Subtype / growth & development
  • Influenza A Virus, H3N2 Subtype / isolation & purification
  • Influenza, Human
  • Madin Darby Canine Kidney Cells / virology*
  • Mutation
  • Orthomyxoviridae / genetics*
  • Orthomyxoviridae / isolation & purification*
  • Receptors, Virus / genetics
  • Receptors, Virus / metabolism
  • Sialyltransferases / genetics
  • Sialyltransferases / metabolism

Substances

  • Antigens, CD
  • Receptors, Virus
  • Sialyltransferases
  • ST6GAL1 protein, human
  • beta-galactoside alpha-2,3-sialyltransferase