A multiphase theory for spreading microbial swarms and films

Elife. 2019 Apr 30;8:e42697. doi: 10.7554/eLife.42697.

Abstract

Bacterial swarming and biofilm formation are collective multicellular phenomena through which diverse microbial species colonize and spread over water-permeable tissue. During both modes of surface translocation, fluid uptake and transport play a key role in shaping the overall morphology and spreading dynamics. Here we develop a generalized two-phase thin-film model that couples bacterial growth, extracellular matrix swelling, fluid flow, and nutrient transport to describe the expansion of both highly motile bacterial swarms, and sessile bacterial biofilms. We show that swarm expansion corresponds to steady-state solutions in a nutrient-rich, capillarity dominated regime. In contrast, biofilm colony growth is described by transient solutions associated with a nutrient-limited, extracellular polymer stress driven limit. We apply our unified framework to explain a range of recent experimental observations of steady and unsteady expansion of microbial swarms and biofilms. Our results demonstrate how the physics of flow and transport in slender geometries serve to constrain biological organization in microbial communities.

Keywords: B. subtilis; biofilms; biophysics; bioswarms; collective spreading; physics of living systems.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Bacillus subtilis / growth & development
  • Bacillus subtilis / physiology
  • Bacteria / growth & development*
  • Bacterial Physiological Phenomena*
  • Biofilms / growth & development*
  • Extracellular Matrix
  • Locomotion / physiology*
  • Models, Biological
  • Osmotic Pressure
  • Surface Properties