nNOS/GSNOR interaction contributes to skeletal muscle differentiation and homeostasis

Cell Death Dis. 2019 May 1;10(5):354. doi: 10.1038/s41419-019-1584-3.

Abstract

Neuronal nitric oxide synthase (nNOS) plays a crucial role in the maintenance of correct skeletal muscle function due, at least in part, to S-nitrosylation of specific protein targets. Similarly, we recently provided evidence for a muscular phenotype in mice lacking the denitrosylase S-nitrosoglutathione reductase (GSNOR). Here, we demonstrate that nNOS and GSNOR are concomitantly expressed during differentiation of C2C12. They colocalizes at the sarcolemma and co-immunoprecipitate in cells and in myofibers. We also provide evidence that GSNOR expression decreases in mouse models of muscular dystrophies and of muscle atrophy and wasting, i.e., aging and amyotrophic lateral sclerosis, suggesting a more general regulatory role of GSNOR in skeletal muscle homeostasis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aging / genetics*
  • Aging / metabolism
  • Alcohol Dehydrogenase / antagonists & inhibitors
  • Alcohol Dehydrogenase / deficiency
  • Alcohol Dehydrogenase / genetics*
  • Animals
  • Cell Differentiation
  • Cell Line, Transformed
  • Disease Models, Animal
  • Dystrophin-Associated Proteins / genetics
  • Dystrophin-Associated Proteins / metabolism
  • Gene Expression Regulation
  • Homeostasis / genetics*
  • Humans
  • Mice
  • Mice, Inbred mdx
  • Mice, Knockout
  • Muscle Development / genetics*
  • Muscle, Skeletal / enzymology
  • Muscle, Skeletal / pathology
  • Muscular Dystrophies / genetics*
  • Muscular Dystrophies / metabolism
  • Muscular Dystrophies / pathology
  • Myoblasts / cytology
  • Myoblasts / enzymology
  • Nitric Oxide / metabolism
  • Nitric Oxide Synthase Type I / genetics*
  • Nitric Oxide Synthase Type I / metabolism
  • RNA, Small Interfering / genetics
  • RNA, Small Interfering / metabolism
  • Sarcolemma / enzymology
  • Signal Transduction
  • Superoxide Dismutase-1 / genetics
  • Superoxide Dismutase-1 / metabolism

Substances

  • Dystrophin-Associated Proteins
  • RNA, Small Interfering
  • syntrophin
  • Nitric Oxide
  • Adh5 protein, mouse
  • Alcohol Dehydrogenase
  • Nitric Oxide Synthase Type I
  • Nos1 protein, mouse
  • Sod1 protein, mouse
  • Superoxide Dismutase-1