Devosia naphthalenivorans sp. nov., isolated from East Pacific Ocean sediment

Abstract

A Gram-stain-negative bacterium, designated CM5-1^T, was isolated from a sediment sample collected from the East Pacific Ocean. 16S rRNA gene sequence analysis revealed that strain CM5-1^T belongs to the genus Devosia, with closely related type strains Devosia submarina KMM 9415^T (98.6 %), Devosia psychrophilaCr7-05^T (98.6 %) and Devosia psychrophilaE84^T (98.2 %). Up-to-date bacterial core gene set analysis revealed that strain CM5-1^T represents one independent lineage with D. submarina KMM 9415^T. The average nucleotide identity values of CM5-1^T with D. submarina KMM 9415^T and D. psychrophila Cr7-05^T are 80.1 and 77.9 %, respectively. In silico DNA-DNA hybridization values between strain CM5-1^T and D. submarina KMM 9415^T and D. psychrophila Cr7-05^T are 23.8 and 21.9 %, respectively. Strain CM5-1^T contains diphosphatidylglycerol, phosphatidylglycerol and glycolipid as major polar lipids. The sole isoprenoid quinone is ubiquinone-10, and C18 : 1ω7c and 11-methyl C18 : 1ω7c are the dominant cellular fatty acids. The G+C content of the genomic DNA is 61.4 mol%. Differential phylogenetic distinctiveness and chemotaxonomic differences, together with the phenotypic properties observed in this study, revealed that strain CM5-1^T could be differentiated from closely related species. Therefore, we propose strain CM5-1^T as a novel species of the genus Devosia, for which the name Devosia naphthalenivorans sp. nov. is suggested. The type strain is CM5-1^T (=JCM32509^T=CGMCC 1.13553^T).

Keywords: 16S rRNA gene; Devosia; average nucleotide identity; in silico DNA–DNA hybridization; up-to-date bacterial core gene.