Thy-1-mediated T-cell Activation Requires Co-Expression of CD3/Ti Complex

Nature. 1987 Apr 2-8;326(6112):505-7. doi: 10.1038/326505a0.

Abstract

In addition to monoclonal antibodies against the CD3 (T3)-T-cell antigen receptor (CD3/Ti) complex, several other monoclonals directed towards distinct cell surface structures on human (CD2 (T11) and Tp44) and murine (Thy-1, TAP, and Ly-6) T lymphocytes are capable of activating T cells. It has been proposed that such structures may function as alternative pathways of stimulation. To examine directly whether any relationship exists between Thy-1-dependent activation phenomena and T-cell activation mediated through the CD3/Ti complex, we have transfected several CD3/Ti- variants of the human T-cell line Jurkat with the murine Thy-1.2 gene. Our data indicate that in CD3/Ti-, Thy-1.2+ transfectants, monoclonal antibodies against Thy-1.2 can induce a rise in cytoplasmic free calcium ([Ca2+]i), but fail to stimulate interleukin-2 (IL-2) production. The only defect in these variant cell lines responsible for the inability to produce IL-2 in response to Thy-1 stimulation was in the expression of the CD3/Ti complex, because replacement of defective Ti alpha- or beta-chain genes reconstributed both surface expression of CD3/Ti and responsiveness to Thy-1 in the IL-2 production assay.

MeSH terms

  • Antigen-Antibody Complex / immunology
  • Calcimycin / pharmacology
  • Cell Line
  • Humans
  • Interleukin-2 / biosynthesis
  • Isoantibodies*
  • Lymphocyte Activation*
  • Receptors, Antigen, T-Cell / immunology*
  • T-Lymphocytes / drug effects
  • T-Lymphocytes / immunology*
  • Tetradecanoylphorbol Acetate / pharmacology

Substances

  • Antigen-Antibody Complex
  • Interleukin-2
  • Isoantibodies
  • Receptors, Antigen, T-Cell
  • anti-Thy antibody
  • Calcimycin
  • Tetradecanoylphorbol Acetate