Silymarin-loaded nanostructured lipid carrier gel for the treatment of skin cancer

Nanomedicine (Lond). 2019 May;14(9):1077-1093. doi: 10.2217/nnm-2018-0235. Epub 2019 May 3.

Abstract

Aim: The present study was aimed at determining the antiproliferative, antioxidant, anti-inflammatory and antitumor activity of developed silymarin-nanostructured lipid carrier (NLC) gel. Materials & methods: B16 melanoma cell line and albino mice were used as ex vivo and in vivo models, respectively, to evaluate the aforementioned pharmacological activities. Results: The volume of large tumors significantly (p < 0.05) reduced from 5.02 to 3.05 mm3, levels of IL-1α and TNF-α were significantly (p < 0.001) lower and levels of superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH) significantly (p < 0.0001) increased in the group treated with silymarin-NLC gel. Furthermore, in skin treated with placebo and conventional gels, a basosquamous carcinoma and squamous cell carcinoma were noticed, respectively. Conclusion: Silymarin-NLC gel presented better treatment outcomes compared with silymarin-conventional gel.

Keywords: antiproliferative activity; antitumor activity; nanomedicine; nanostructured lipid carrier (NLC); oxidative stress; silymarin; silymarin-NLC gel; skin cancer; topical drug delivery; tumor burden.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antineoplastic Agents / administration & dosage
  • Antineoplastic Agents / therapeutic use*
  • Carcinogenesis / drug effects
  • Carcinogenesis / pathology
  • Catalase / metabolism
  • Cell Line, Tumor
  • Cell Proliferation
  • Cell Survival / drug effects
  • Female
  • Gels
  • Glutathione / metabolism
  • Humans
  • Lipids / chemistry*
  • Male
  • Malondialdehyde / metabolism
  • Melanoma, Experimental / drug therapy*
  • Melanoma, Experimental / pathology
  • Mice
  • Nanocapsules / chemistry*
  • Silymarin / administration & dosage
  • Silymarin / therapeutic use*
  • Skin Neoplasms / drug therapy*
  • Skin Neoplasms / pathology
  • Superoxide Dismutase / metabolism
  • Treatment Outcome

Substances

  • Antineoplastic Agents
  • Gels
  • Lipids
  • Nanocapsules
  • Silymarin
  • Malondialdehyde
  • Catalase
  • Superoxide Dismutase
  • Glutathione