Effect of Ischemic Preconditioning and Postconditioning on Exosome-Rich Fraction microRNA Levels, in Relation with Electrophysiological Parameters and Ventricular Arrhythmia in Experimental Closed-Chest Reperfused Myocardial Infarction

Int J Mol Sci. 2019 Apr 30;20(9):2140. doi: 10.3390/ijms20092140.


We investigated the antiarrhythmic effects of ischemic preconditioning (IPC) and postconditioning (PostC) by intracardiac electrocardiogram (ECG) and measured circulating microRNAs (miRs) that are related to cardiac conduction. Domestic pigs underwent 90-min. percutaneous occlusion of the mid left anterior coronary artery, followed by reperfusion. The animals were divided into three groups: acute myocardial infarction (AMI, n = 7), ischemic preconditioning-acute myocardial infarction (IPC-AMI) (n = 9), or AMI-PostC (n = 5). IPC was induced by three 5-min. episodes of repetitive ischemia/reperfusion cycles (rI/R) before AMI. PostC was induced by six 30-s rI/R immediately after induction of reperfusion 90 min after occlusion. Before the angiographic procedure, a NOGA endocardial mapping catheter was placed again the distal anterior ventricular endocardium to record the intracardiac electrogram (R-amplitude, ST-Elevation, ST-area under the curve (AUC), QRS width, and corrected QT time (QTc)) during the entire procedure. An arrhythmia score was calculated. Cardiac MRI was performed after one-month. IPC led to significantly lower ST-elevation, heart rate, and arrhythmia score during ischemia. PostC induced a rapid recovery of R-amplitude, decrease in QTc, and lower arrhythmia score during reperfusion. Slightly higher levels of miR-26 and miR-133 were observed in AMI compared to groups IPC-AMI and AMI-PostC. Significantly lower levels of miR-1, miR-208, and miR-328 were measured in the AMI-PostC group as compared to animals in group AMI and IPC-AMI. The arrhythmia score was not significantly associated with miRNA plasma levels. Cardiac MRI showed significantly smaller infarct size in the IPC-AMI group when compared to the AMI and AMI-PostC groups. Thus, IPC led to better left ventricular ejection fraction at one-month and it exerted antiarrhythmic effects during ischemia, whereas PostC exhibited antiarrhythmic properties after reperfusion, with significant downregulaton of ischemia-related miRNAs.

Keywords: acute myocardial infarction; cardiac electrophysiology; electrocardiography; exosome-rich fraction; exosomes; extracellular vesicles; ischemia-reperfusion injury; ischemic postconditioning; ischemic preconditioning; miRNA; reperfusion arrhythmia; ventricular arrhythmia.

MeSH terms

  • Animals
  • Exosomes / metabolism*
  • Female
  • Heart Ventricles / metabolism
  • Ischemic Postconditioning*
  • Ischemic Preconditioning, Myocardial*
  • MicroRNAs / genetics
  • MicroRNAs / metabolism*
  • Myocardial Infarction / complications
  • Myocardial Infarction / metabolism*
  • Myocardial Infarction / therapy
  • Swine
  • Ventricular Fibrillation / etiology
  • Ventricular Fibrillation / metabolism*
  • Ventricular Fibrillation / therapy
  • Ventricular Function


  • MicroRNAs